Standard Curve (Sample)
Human Squalene Monooxygenase (SQLE) ELISA Kit | SQLE elisa kit
Human Squalene Monooxygenase (SQLE) ELISA Kit
Reactivity
Human
Synonyms
Squalene Monooxygenase (SQLE); N/A; Human Squalene Monooxygenase (SQLE) ELISA Kit; SQLE; FLJ30795; squalene monooxygenase; SQLE elisa kit
Reactivity
Human
Specificity
Human Squalene monooxygenase (SQLE) ELISA Kit has high sensitivity and excellent specificity for detection of Human SQLE. No significant cross-reactivity or interference between Human SQLE and analogues was observed.
Sequence Length
574
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
10 pg/ml - 160 pg/ml
Sensitivity
< 1pg/ml
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SQLE elisa kit
Background: Squalene epoxidase (EC 1.14.99.7) catalyzes the first oxygenation step in sterol biosynthesis and is thought to be one of the rate-limiting enzymes in this pathway. Squalene epoxidase (SE) is a key flavin adenine dinucleotide (FAD)-dependent enzyme of ergosterol and cholesterol biosynthetic pathways and an attractive potential target for drugs used to inhibit the growth of pathogenic fungi or to lower cholesterol level. By PCR analysis of a human/rodent somatic cell hybrid mapping panel, Nagai et al. (1997) demonstrated that the human SQLE gene maps to chromosome 8. The localization was refined by PCR analysis of a radiation hybrid panel. The results showed that human SQLE is most tightly linked to D8S508, which is reported to be located at 8q24.13-qter. The authors used fluorescence in situ hybridization to map SQLE to 8q24.1.
Principle of the Assay: Human Squalene monooxygenase (SQLE) ELISA Kit employs a two-site sandwich ELISA to quantitate SQLE in samples. An antibody specific for SQLE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SQLE present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SQLE detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SQLE bound in the initial step.
Principle of the Assay: Human Squalene monooxygenase (SQLE) ELISA Kit employs a two-site sandwich ELISA to quantitate SQLE in samples. An antibody specific for SQLE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SQLE present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human SQLE detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of SQLE bound in the initial step.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
squalene monooxygenase
NCBI Official Synonym Full Names
squalene epoxidase
NCBI Official Symbol
SQLE
NCBI Protein Information
squalene monooxygenase
UniProt Protein Name
Squalene monooxygenase
UniProt Gene Name
SQLE
UniProt Synonym Gene Names
ERG1; SE
UniProt Entry Name
ERG1_HUMAN
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Product Notes
The Human SQLE sqle (Catalog #AAA330570) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330570 ELISA Kit recognizes Human SQLE. It is sometimes possible for the material contained within the vial of "Squalene Monooxygenase (SQLE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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