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product-image-AAA322890_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from rat muscle, using SQLE antibody. Lane 1 was treated with the blocking peptide.)

Rabbit SQLE Polyclonal Antibody | anti-SQLE antibody

SQLE Antibody

Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
SQLE, Antibody; SQLE Antibody; ERG1; ERG1_HUMAN; SE; SQLE; Squalene epoxidase; Squalene monooxygenase; anti-SQLE antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SQLE antibody detects endogenous levels of SQLE.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
574
Applicable Applications for anti-SQLE antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human SQLE.
Subcellular Location
Microsome membrane. Endoplasmic Reticulum Membrane.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (88%), Bovine (100%), Horse (83%), Sheep (100%), Rabbit (100%), Dog (92%), Chicken (83%), Xenopus (83%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts from rat muscle, using SQLE antibody. Lane 1 was treated with the blocking peptide.)

product-image-AAA322890_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from rat muscle, using SQLE antibody. Lane 1 was treated with the blocking peptide.)

WB (Western Blot)

(Western blot analysis of extracts of A549 cells, using SQLE antibody.)

product-image-AAA322890_WB13.jpg WB (Western Blot) (Western blot analysis of extracts of A549 cells, using SQLE antibody.)

IHC (Immunohistochemistry)

(AAA322890 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

product-image-AAA322890_IHC15.jpg IHC (Immunohistochemistry) (AAA322890 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-SQLE antibody
Function: Catalyzes the first oxygenation step in sterol biosynthesis and is suggested to be one of the rate-limiting enzymes in this pathway.
Subunit Structure: May form a complex with squalene synthase.
Similarity: Belongs to the squalene monooxygenase family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 64 kDa
Predicted: 64 kDa
NCBI Official Full Name
squalene monooxygenase
NCBI Official Synonym Full Names
squalene epoxidase
NCBI Official Symbol
SQLE
NCBI Protein Information
squalene monooxygenase
UniProt Protein Name
Squalene monooxygenase
UniProt Gene Name
SQLE
UniProt Synonym Gene Names
ERG1; SE

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Product Notes

The SQLE sqle (Catalog #AAA322890) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SQLE Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SQLE can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SQLE sqle for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SQLE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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