Human T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) ELISA Kit | TIGIT elisa kit

Human T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) ELISA Kit

Cat. Num. AAA140547

• Gene Names: TIGIT; VSIG9; VSTM3; WUCAM
• Reactivity: Human
• Synonyms: T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT); N/A; Human T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) ELISA Kit; VSIG9; VSTM3; V-Set And Immunoglobulin Domain Containing 9; V-Set And Transmembrane Domain Containing 3; TIGIT elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT).; No significant cross-reactivity or interference between T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) and analogues was observed.
• Sequence Length: 244
• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.114ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for TIGIT elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell Immunoreceptor With Ig And ITIM Domains Protein (TIGIT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TIGIT elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 256600228
• NCBI GeneID: 201633
• NCBI Accession #: NP_776160.2
• NCBI GenBank Nucleotide #: NM_173799.3
• Molecular Weight: 18,432 Da
• NCBI Official Full Name: T-cell immunoreceptor with Ig and ITIM domains
• NCBI Official Synonym Full Names: T cell immunoreceptor with Ig and ITIM domains
• NCBI Official Symbol: TIGIT
• NCBI Official Synonym Symbols: VSIG9; VSTM3; WUCAM
• NCBI Protein Information: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Protein Name: T-cell immunoreceptor with Ig and ITIM domains
• UniProt Gene Name: TIGIT
• UniProt Synonym Gene Names: VSIG9; VSTM3
• UniProt Entry Name: TIGIT_HUMAN