Standard Curve (Sample)
(Fig.1. Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) Standard Curve.)
Human T Cell Immunoreceptor With Ig And ITIM Domains (TIGIT) ELISA Kit | TIGIT elisa kit
Human T Cell Immunoreceptor With Ig And ITIM Domains (TIGIT) ELISA Kit
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human
Synonyms
T Cell Immunoreceptor With Ig And ITIM Domains (TIGIT); N/A; Human T Cell Immunoreceptor With Ig And ITIM Domains (TIGIT) ELISA Kit; DKFZp667A205; FLJ39873; VSIG9; VSTM3; WUCAM; V-set and immunoglobulin domain containing 9; V-set and transmembrane domain containing 3; Washington University cell adhesion molecule; TIGIT elisa kit
Reactivity
Human
Specificity
Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIGIT. No significant cross-reactivity or interference between Human TIGIT and analogues was observed.
Sequence Length
244
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) Standard Curve.)
Related Product Information for TIGIT elisa kit
Description: This Human T cell immunoreceptor with Ig and ITIM domains (TIGIT) ELISA Kit employs a two-site sandwich ELISA to quantitate TIGIT in samples. An antibody specific for TIGIT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIGIT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIGIT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIGIT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TIGIT encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. The predicted 244-amino acid TIGIT protein has a single Ig domain, followed by a type I transmembrane domain and a single intracellular immunoreceptor tyrosine-based inhibitory motif. It shares 88%, 67%, and 58% amino acid identity with its rhesus, dog, and mouse homologs, respectively.
Background: TIGIT encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. The predicted 244-amino acid TIGIT protein has a single Ig domain, followed by a type I transmembrane domain and a single intracellular immunoreceptor tyrosine-based inhibitory motif. It shares 88%, 67%, and 58% amino acid identity with its rhesus, dog, and mouse homologs, respectively.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
TIGIT
NCBI Official Synonym Symbols
VSIG9; VSTM3; WUCAM
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
UniProt Gene Name
TIGIT
UniProt Synonym Gene Names
VSIG9; VSTM3
Similar Products
Product Notes
The Human TIGIT tigit (Catalog #AAA331975) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331975 ELISA Kit recognizes Human TIGIT. It is sometimes possible for the material contained within the vial of "T Cell Immunoreceptor With Ig And ITIM Domains (TIGIT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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