Standard Curve (Sample)
(Fig.1. Human Tenascin-C (TNC) Standard Curve.)
Human Tenascin-C (TNC) ELISA Kit | TNC elisa kit
Human Tenascin-C (TNC) ELISA Kit
Gene Names
TNNC1; TNC; TN-C; TNNC; CMD1Z; CMH13
Reactivity
Human
Synonyms
Tenascin-C (TNC); N/A; Human Tenascin-C (TNC) ELISA Kit; TNNC1; CMD1Z; TNC; TNNC; cardiac troponin C; slow twitch skeletal/cardiac muscle troponin C; troponin C; slow; troponin C1; TNC elisa kit
Reactivity
Human
Specificity
Human Tenascin-C (TNC) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNNC1. No significant cross-reactivity or interference between Human TNNC1 and analogues was observed.
Sequence Length
161
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Tenascin-C (TNC) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Tenascin-C (TNC) Standard Curve.)
Related Product Information for TNC elisa kit
Description: This Human Tenascin-C (TNC) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNC1 in samples. An antibody specific for TNNC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tenascinis an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexomeric, multidomain protein with disulfide-linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia, in tendon anlagen, and in developing cartilage and bone. In the adult tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Pearson et al. (1988) isolated cDNA clones coding for tenascin from a chicken fibroblast cDNA expression library using a specific tenascin antiserum. They showed induction of tenascin in vitro by fetal calf serum as well as by transforming growth factor-beta.
Background: Tenascinis an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexomeric, multidomain protein with disulfide-linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia, in tendon anlagen, and in developing cartilage and bone. In the adult tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle. Pearson et al. (1988) isolated cDNA clones coding for tenascin from a chicken fibroblast cDNA expression library using a specific tenascin antiserum. They showed induction of tenascin in vitro by fetal calf serum as well as by transforming growth factor-beta.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Troponin C, slow skeletal and cardiac muscles
NCBI Official Synonym Full Names
troponin C1, slow skeletal and cardiac type
NCBI Official Symbol
TNNC1
NCBI Official Synonym Symbols
TNC; TN-C; TNNC; CMD1Z; CMH13
NCBI Protein Information
troponin C, slow skeletal and cardiac muscles
UniProt Protein Name
Troponin C, slow skeletal and cardiac muscles
UniProt Gene Name
TNNC1
UniProt Synonym Gene Names
TNNC; TN-C
UniProt Entry Name
TNNC1_HUMAN
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Product Notes
The Human TNC tnnc1 (Catalog #AAA331974) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331974 ELISA Kit recognizes Human TNC. It is sometimes possible for the material contained within the vial of "Tenascin-C (TNC), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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