Human Tetranectin/CLEC3B ELISA Kit | CLEC3B elisa kit

Human Tetranectin/CLEC3B PicoKine ELISA Kit

Cat. Num. AAA45994

• Gene Names: CLEC3B; TN; TNA
• Reactivity: Human
• Synonyms: Tetranectin/CLEC3B; N/A; Human Tetranectin/CLEC3B PicoKine ELISA Kit; Tetranectin; C-type lectin domain family 3, member B; C type lectin domain family 3 member B; C-type lectin domain family 3 member B; Clec3b; Plasminogen kringle 4 binding protein; Plasminogen kringle 4-binding protein; TETN_HUMAN; Tetranectin (plasminogen binding protein); TN; TNA; CLEC3B elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Tetranectin
• Sequence Length: 202
• Samples: Cell culture supernates, serum and tissue Homogenates
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human Tetranectin/CLEC3B PicoKine ELISA Kit standard curve)

Related Product Information for CLEC3B elisa kit

Principle of the assay: human Tetranectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Tetranectin has been precoated onto 96-well plates. Standards(NSO, E22-V202) and test samples are added to the wells; a biotinylated detection polyclonal antibody from goat specific for Tetranectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Tetranectin amount of sample captured in plate.
Background: Tetranectin, also called TNA, is a protein that in humans is encoded by the CLEC3B gene. It is mapped to 3p21.31. Tetranectin, a tetrameric protein isolated from human plasma, has 4 identical and noncovalently bound polypeptide chains, each of 181 amino acid residues. It has a specific binding affinity for sulfated polysaccharides and the kringle 4 of plasminogen. The plasma concentration of tetranectin is reduced in patients with various malignancies. Tetranectin is a plasminogen-binding protein that is induced during the mineralization phase of osteogenesis. Thus, tetranectin is a candidate gene for human disorders affecting bone and connective tissue.

NCBI and Uniprot Product Information

• NCBI GI #: 317373499
• NCBI GeneID: 7123
• UniProt Accession #: P05452; Q6FGX6
• Molecular Weight: 22,537 Da
• NCBI Official Full Name: Tetranectin
• NCBI Official Synonym Full Names: C-type lectin domain family 3, member B
• NCBI Official Symbol: CLEC3B
• NCBI Official Synonym Symbols: TN; TNA
• NCBI Protein Information: tetranectin; plasminogen kringle 4-binding protein; tetranectin (plasminogen binding protein); tetranectin (plasminogen-binding protein)
• UniProt Protein Name: Tetranectin
• UniProt Gene Name: CLEC3B
• UniProt Synonym Gene Names: TNA; TN
• UniProt Entry Name: TETN_HUMAN