Standard Curve (Sample)
Human Tetraspanin 25 (TSPAN25) ELISA Kit | TSPAN25 elisa kit
Human Tetraspanin 25 (TSPAN25) ELISA Kit
Gene Names
CD53; MOX44; TSPAN25
Reactivity
Human
Synonyms
Tetraspanin 25 (TSPAN25); N/A; Human Tetraspanin 25 (TSPAN25) ELISA Kit; CD53; MOX44; TSPAN25; Leukocyte surface antigen CD53; Cell surface glycoprotein CD53; TSPAN25/Tetraspanin 25; TSPAN25 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of TSPAN25. No significant cross-reactivity or interference between TSPAN25 and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates, cell lysates and other biological fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.125ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TSPAN25 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TSPAN25 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (within shelf life)
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Shelf Life: 8 months
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TSPAN25 elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TSPAN25. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TSPAN25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TSPAN25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TSPAN25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TSPAN25 in Human tissue homogenates, cell lysates and other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TSPAN25 in Human tissue homogenates, cell lysates and other biological fluids.
Product Categories/Family for TSPAN25 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,341 Da
NCBI Official Full Name
leukocyte surface antigen CD53
NCBI Official Synonym Full Names
CD53 molecule
NCBI Official Symbol
CD53
NCBI Official Synonym Symbols
MOX44; TSPAN25
NCBI Protein Information
leukocyte surface antigen CD53; CD53 antigen; CD53 glycoprotein; CD53 tetraspan antigen; antigen MOX44 identified by monoclonal antibody MRC-OX44; cell surface antigen; cell surface glycoprotein CD53; tetraspanin-25; transmembrane glycoprotein; tspan-25
UniProt Protein Name
Leukocyte surface antigen CD53
UniProt Gene Name
CD53
UniProt Synonym Gene Names
MOX44; TSPAN25; Tspan-25
UniProt Entry Name
CD53_HUMAN
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Product Notes
The Human TSPAN25 cd53 (Catalog #AAA189750) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA189750 ELISA Kit recognizes Human TSPAN25. It is sometimes possible for the material contained within the vial of "Tetraspanin 25 (TSPAN25), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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