Application Data
(This assay recognizes both recombinant and natural Human TREML1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human TLT-1/TREML1 ELISA Kit | TLT-1/TREML1 elisa kit
Human TLT-1/TREML1 ELISA Kit
Reactivity
Human
Synonyms
TLT-1/TREML1; N/A; Human TLT-1/TREML1 ELISA Kit; TLT-1/TREML1 ELISA Kit, Human; triggering receptor expressed on myeloid cells like 1; dJ238O23.3 ELISA Kit, Human; GLTL1825 ELISA Kit, Human; MGC119173 ELISA Kit, Human; PRO3438 ELISA Kit, Human; TLT-1 ELISA Kit, Human; TLT1 ELISA Kit, Human; UNQ1825/PRO3438 ELISA Kit, Human; TLT-1/TREML1 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human TLT-1/TREML1 concentration in serum, cell culture supernates and plasma.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum, Cell supernatant, Plasma
Detection Range
28.13-1800pg/mL
Sensitivity
12.98pg/mL
Conjugation
HRP
Interference
Preparations of the factors listed below at 50ng/mL in a mid-range recombinant Human TREML1 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human TREML1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Human TREML1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for TLT-1/TREML1 elisa kit
This TLT-1/TREML1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TLT-1/TREML1 protein in Serum, Cell supernatant, Plasma. It contains recombinant Human TLT-1/TREML1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Trem-like transcript 1 protein, also known as Triggering receptor expressed on myeloid cells-like protein 1, TREML1 and TLT-1, is a cytoplasm and single-pass type I membrane protein. TREML1/TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that its expression is up-regulated dramatically upon platelet activation. It is a receptor that may play a role in the innate and adaptive immune response. TREML1/TLT-1 contains the characteristic single V-set immunoglobulin (Ig) domain, its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif, the latter known to be used for interactions with protein tyrosine phosphatases. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system, including neutrophils, monocyte/macrophages, and dendritic cells, via their association with DAP12. TREML1/TLT-1 is prepackaged, along with CD62P, into both MK and platelet alpha-granules. Differences in thrombin-induced redistribution of CD62P and TREML1 indicate that TREML1 is not simply cargo of alpha-granules but may instead regulate granule construction or dispersal. TREML1/TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury.
Background: Trem-like transcript 1 protein, also known as Triggering receptor expressed on myeloid cells-like protein 1, TREML1 and TLT-1, is a cytoplasm and single-pass type I membrane protein. TREML1/TLT-1 is expressed exclusively in platelets and megakaryocytes (MKs) and that its expression is up-regulated dramatically upon platelet activation. It is a receptor that may play a role in the innate and adaptive immune response. TREML1/TLT-1 contains the characteristic single V-set immunoglobulin (Ig) domain, its longer cytoplasmic tail is composed of both a proline-rich region and an immune receptor tyrosine-based inhibitory motif, the latter known to be used for interactions with protein tyrosine phosphatases. The triggering receptors expressed on myeloid cells (TREMs) have drawn considerable attention due to their ability to activate multiple cell types within the innate immune system, including neutrophils, monocyte/macrophages, and dendritic cells, via their association with DAP12. TREML1/TLT-1 is prepackaged, along with CD62P, into both MK and platelet alpha-granules. Differences in thrombin-induced redistribution of CD62P and TREML1 indicate that TREML1 is not simply cargo of alpha-granules but may instead regulate granule construction or dispersal. TREML1/TLT-1 does not function to inhibit members of the TREM family but instead may play a role in maintaining vascular hemostasis and regulating coagulation and inflammation at sites of injury.
References
Washington A.V., et al., 2002, Blood 100:3822-4.
Allcock R.J.N., et al., 2003, Eur. J. Immunol. 33:567-77.
Washington A.V., et al., 2004, Blood 104:1042-7.
Barrow A.D., et al., 2004, J. Immunol. 172:5838-42.
Gattis J.L., et al., 2006, J. Biol. Chem. 281:13396-403.
Allcock R.J.N., et al., 2003, Eur. J. Immunol. 33:567-77.
Washington A.V., et al., 2004, Blood 104:1042-7.
Barrow A.D., et al., 2004, J. Immunol. 172:5838-42.
Gattis J.L., et al., 2006, J. Biol. Chem. 281:13396-403.
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Product Notes
The Human TLT-1/TREML1 (Catalog #AAA258441) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258441 ELISA Kit recognizes Human TLT-1/TREML1. It is sometimes possible for the material contained within the vial of "TLT-1/TREML1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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