Standard Curve (Sample)
(Fig.1. Human TNF receptor-associated factor 3 (TRAF3) Standard Curve.)
Human TNF Receptor-Associated Factor 3 (TRAF3) ELISA Kit | TRAF3 elisa kit
Human TNF Receptor-Associated Factor 3 (TRAF3) ELISA Kit
Gene Names
TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp; RNF118
Reactivity
Human
Synonyms
TNF Receptor-Associated Factor 3 (TRAF3); N/A; Human TNF Receptor-Associated Factor 3 (TRAF3) ELISA Kit; TRAF3; CAP-1; CD40bp; CRAF1; LAP1; CD40 associated protein 1; CD40 binding protein; CD40 receptor associated factor 1; LMP1 associated protein; TRAF3 elisa kit
Reactivity
Human
Specificity
Human TNF receptor-associated factor 3 (TRAF3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRAF3. No significant cross-reactivity or interference between Human TRAF3 and analogues was observed.
Sequence Length
568
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human TNF receptor-associated factor 3 (TRAF3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human TNF receptor-associated factor 3 (TRAF3) Standard Curve.)
Related Product Information for TRAF3 elisa kit
Description: This Human TNF receptor-associated factor 3 (TRAF3) ELISA Kit employs a two-site sandwich ELISA to quantitate TRAF3 in samples. An antibody specific for TRAF3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRAF3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAF3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAF3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TRAF3 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
Background: TRAF3 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
TNF receptor-associated factor 3
NCBI Official Synonym Full Names
TNF receptor associated factor 3
NCBI Official Symbol
TRAF3
NCBI Official Synonym Symbols
CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp; RNF118
NCBI Protein Information
TNF receptor-associated factor 3
UniProt Protein Name
TNF receptor-associated factor 3
UniProt Gene Name
TRAF3
UniProt Synonym Gene Names
CAP1; CRAF1; CRAF1; CD40BP; LAP1
UniProt Entry Name
TRAF3_HUMAN
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Product Notes
The Human TRAF3 traf3 (Catalog #AAA330363) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330363 ELISA Kit recognizes Human TRAF3. It is sometimes possible for the material contained within the vial of "TNF Receptor-Associated Factor 3 (TRAF3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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