Human Transcription factor MafK (MAFK) ELISA Kit | MAFK elisa kit

Human Transcription factor MafK (MAFK) ELISA Kit

Cat. Num. AAA245254

• Gene Names: MAFK; P18; NFE2U
• Reactivity: Human
• Synonyms: Transcription factor MafK (MAFK); N/A; Human Transcription factor MafK (MAFK) ELISA Kit; MAFK elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAFK. No significant cross-reactivity or interference between MAFK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAFK and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MAFK elisa kit

Principle of the Assay: MAFK ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MAFK antibody and an MAFK-HRP conjugate. The assay sample and buffer are incubated together with MAFK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAFK concentration since MAFK from samples and MAFK-HRP conjugate compete for the anti-MAFK antibody binding site. Since the number of sites is limited, as more sites are occupied by MAFK from the sample, fewer sites are left to bind MAFK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAFK concentration in each sample is interpolated from this standard curve.

Intended Uses: This MAFK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human MAFK. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for MAFK elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 4505075
• NCBI GeneID: 7975
• NCBI Accession #: NP_002351.1
• NCBI GenBank Nucleotide #: NM_002360.3
• Molecular Weight: 17,523 Da
• NCBI Official Full Name: transcription factor MafK
• NCBI Official Synonym Full Names: v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog K
• NCBI Official Symbol: MAFK
• NCBI Official Synonym Symbols: P18; NFE2U
• NCBI Protein Information: transcription factor MafK; nuclear factor erythroid-2, ubiquitous (p18); basic-leucine zipper transcription factor MafK; erythroid transcription factor NF-E2 p18 subunit; v-maf musculoaponeurotic fibrosarcoma oncogene homolog K; v-maf avian musculoaponeurotic fibrosarcoma oncogene family, protein K
• UniProt Protein Name: Transcription factor MafK
• UniProt Gene Name: MAFK
• UniProt Entry Name: MAFK_HUMAN