Standard Curve (Sample)
(Fig.1. Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) Standard Curve.)
Human Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6) ELISA Kit | TNFAIP6 elisa kit
Human Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6) ELISA Kit
Gene Names
TNFAIP6; TSG6; TSG-6
Reactivity
Human
Synonyms
Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6); N/A; Human Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6) ELISA Kit; TNFAIP6; TSG-6; TSG6; hyaluronate-binding protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein; TNFAIP6 elisa kit
Reactivity
Human
Specificity
Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFAIP6. No significant cross-reactivity or interference between Human TNFAIP6 and analogues was observed.
Sequence Length
277
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) Standard Curve.)
Related Product Information for TNFAIP6 elisa kit
Description: This Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP6 in samples. An antibody specific for TNFAIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFAIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TSG-6 is a 30 kDa secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IalphaI), and thus enhance the serine protease inhibitory activity of IalphaI, which is important in the protease network associated with inflammation. The expression of this gene can be induced by a number of signalling molecules, principally tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1). The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.
Background: TSG-6 is a 30 kDa secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IalphaI), and thus enhance the serine protease inhibitory activity of IalphaI, which is important in the protease network associated with inflammation. The expression of this gene can be induced by a number of signalling molecules, principally tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1). The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor-inducible gene 6 protein
NCBI Official Synonym Full Names
TNF alpha induced protein 6
NCBI Official Symbol
TNFAIP6
NCBI Official Synonym Symbols
TSG6; TSG-6
NCBI Protein Information
tumor necrosis factor-inducible gene 6 protein
UniProt Protein Name
Tumor necrosis factor-inducible gene 6 protein
UniProt Gene Name
TNFAIP6
UniProt Synonym Gene Names
TSG6; TSG-6; TNF alpha-induced protein 6
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Product Notes
The Human TNFAIP6 tnfaip6 (Catalog #AAA330452) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330452 ELISA Kit recognizes Human TNFAIP6. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor-Inducible Gene 6 Protein (TNFAIP6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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