Standard Curve (Sample)
Human TWEAK ELISA Kit | TWEAK elisa kit
Human TWEAK ELISA Kit
Gene Names
TNFSF12; APO3L; DR3LG; TWEAK; TNLG4A
Reactivity
Human
Synonyms
TWEAK; N/A; Human TWEAK ELISA Kit; TWEAK elisa kit
Reactivity
Human
Specificity
This assay recognizes both recombinant and natural human TWEAK. The factors listed below were prepared at 50ng/ml and assayed for cross-reactivity. No significant cross-reactivity was observed with the following. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between TWEAK and all the analogues, therefore, cross reaction may still exist.
Sequence Length
249
Assay Type
Quantitative Sandwich
Sensitivity
< 7.8pg/mL
Intra-assay Precision
Intra-plate Precision The three samples with known concentrations were repeated 20 times on the same board to calculate the coefficient of variation (CV) for the concentrations. Intra-Assay: CV<10%
Inter-assay Precision
Inter-plate Precision The coefficient of variation (CV) of three known concentrations was calculated by 20 tests of two kits on each of the three samples. Inter-Assay: CV<15%
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TWEAK elisa kit
Background: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TWEAK in human serum, plasma, cell culture supernatants and other biological fluids.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human TWEAK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TWEAK present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated detection antibody specific for human TWEAK is added to the wells. After incubating, an enzyme-linked streptavidin is added and binds to biotin. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TWEAK bound in the initial step. The color development is stopped, and the absorbance is measured.
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for human TWEAK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TWEAK present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-conjugated detection antibody specific for human TWEAK is added to the wells. After incubating, an enzyme-linked streptavidin is added and binds to biotin. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TWEAK bound in the initial step. The color development is stopped, and the absorbance is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,589 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 12 proprotein
NCBI Official Synonym Full Names
TNF superfamily member 12
NCBI Official Symbol
TNFSF12
NCBI Official Synonym Symbols
APO3L; DR3LG; TWEAK; TNLG4A
NCBI Protein Information
tumor necrosis factor ligand superfamily member 12
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 12
UniProt Gene Name
TNFSF12
UniProt Synonym Gene Names
APO3L; DR3LG; TWEAK
Similar Products
Product Notes
The Human TWEAK tnfsf12 (Catalog #AAA281373) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281373 ELISA Kit recognizes Human TWEAK. It is sometimes possible for the material contained within the vial of "TWEAK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.