Standard Curve (Sample)
(Fig.1. Human Ubiquitin-conjugating enzyme E2 K (UBE2K) Standard Curve.)
Human Ubiquitin-Conjugating Enzyme E2 K (UBE2K) ELISA Kit | UBE2K elisa kit
Human Ubiquitin-Conjugating Enzyme E2 K (UBE2K) ELISA Kit
Gene Names
UBE2K; LIG; HIP2; HYPG; UBC1; E2-25K
Reactivity
Human
Synonyms
Ubiquitin-Conjugating Enzyme E2 K (UBE2K); N/A; Human Ubiquitin-Conjugating Enzyme E2 K (UBE2K) ELISA Kit; UBE2K; DKFZp564C1216; DKFZp686J24237; E2-25K; HIP2; HYPG; LIG; huntingtin interacting protein 2; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2K; ubiquitin-p; UBE2K elisa kit
Reactivity
Human
Specificity
Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBE2K. No significant cross-reactivity or interference between Human UBE2K and analogues was observed.
Sequence Length
200
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ubiquitin-conjugating enzyme E2 K (UBE2K) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ubiquitin-conjugating enzyme E2 K (UBE2K) Standard Curve.)
Related Product Information for UBE2K elisa kit
Description: This Human Ubiquitin-conjugating enzyme E2 K (UBE2K) ELISA Kit employs a two-site sandwich ELISA to quantitate UBE2K in samples. An antibody specific for UBE2K has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBE2K present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBE2K is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBE2K bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. The 200-amino acid HIP2 protein sequence was identical to that of bovine ubiquitin-conjugating enzyme E2-25K. Assays of the interaction of HIP2 with huntingtin revealed that HIP2 binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. Western blot analysis detected protein bands of 25, 28, and 45 kD; the 28-kD band appeared to be selective for tissues of the central nervous system.
Background: HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. The 200-amino acid HIP2 protein sequence was identical to that of bovine ubiquitin-conjugating enzyme E2-25K. Assays of the interaction of HIP2 with huntingtin revealed that HIP2 binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. Western blot analysis detected protein bands of 25, 28, and 45 kD; the 28-kD band appeared to be selective for tissues of the central nervous system.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Ubiquitin-conjugating enzyme E2 K
NCBI Official Synonym Full Names
ubiquitin conjugating enzyme E2 K
NCBI Official Symbol
UBE2K
NCBI Official Synonym Symbols
LIG; HIP2; HYPG; UBC1; E2-25K
NCBI Protein Information
ubiquitin-conjugating enzyme E2 K
UniProt Protein Name
Ubiquitin-conjugating enzyme E2 K
UniProt Gene Name
UBE2K
UniProt Synonym Gene Names
HIP2; LIG; HIP-2; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K
UniProt Entry Name
UBE2K_HUMAN
Similar Products
Product Notes
The Human UBE2K ube2k (Catalog #AAA330324) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330324 ELISA Kit recognizes Human UBE2K. It is sometimes possible for the material contained within the vial of "Ubiquitin-Conjugating Enzyme E2 K (UBE2K), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.