Application Data
(AMP-Glo assay for UBE2K activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2K in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
UBE2K recombinant protein
Recombinant UBE2K protein
Purity
>=95% by SDS-PAGE.
Synonyms
UBE2K; N/A; Recombinant UBE2K protein; Ubiquitin conjugating enzyme E2 K; E2-25K; HIP2; HYPG; LIG; UBC1; UBE2K recombinant protein
Host
E coli
Purity/Purification
>=95% by SDS-PAGE.
Form/Format
Recombinant UBE2K protein is supplied in 25 mM Tris 8.0, 300mM NaCl, 20% glycerol, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant UBE2K protein that includes full length of human UBE2K protein (accession number NP_005330.1) was expressed in E coli and contains an N-terminal His tag with a molecular weight of 24.57kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(AMP-Glo assay for UBE2K activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2K in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
Application Data
(AMP-Glo assay for UBE2K activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2K in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence were read after another 30 min incubation.)
SDS-PAGE
(Recombinant UBE2K protein gel. 12.5% SDS-PAGE Coomassie blue MW: 24.57kDa Purity: >95%)
SDS-PAGE
(Recombinant UBE2K protein gel. 12.5% SDS-PAGE Coomassie blue MW: 24.57kDa Purity: >95%)
Related Product Information for UBE2K recombinant protein
UBE2K (Ubiquitin-conjugating enzyme E2 K) also known as E2-25K, HIP2, HYPG, LIG and UBC1. UBE2K is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2K can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin protein ligase complex, catalyzes the synthesis of ‘Lys-48’-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded luminal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein-dependent degradation of RB1.
Product Categories/Family for UBE2K recombinant protein
Similar Products
Product Notes
The UBE2K (Catalog #AAA60391) is a Recombinant Protein produced from E coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "UBE2K, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.