Standard Curve (Sample)
(Fig.1. Human Ubiquitin D (UBD) Standard Curve.)
Human Ubiquitin D (UBD) ELISA Kit | UBD elisa kit
Human Ubiquitin D (UBD) ELISA Kit
Gene Names
UBD; FAT10; UBD-3; GABBR1
Reactivity
Human
Synonyms
Ubiquitin D (UBD); N/A; Human Ubiquitin D (UBD) ELISA Kit; UBD; DADB-115L1.4; FAT10; GABBR1; UBD-3; diubiquitin; UBD elisa kit
Reactivity
Human
Specificity
Human Ubiquitin D (UBD) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBD. No significant cross-reactivity or interference between Human UBD and analogues was observed.
Sequence Length
165
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Ubiquitin D (UBD) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Ubiquitin D (UBD) Standard Curve.)
Related Product Information for UBD elisa kit
Description: This Human Ubiquitin D (UBD) ELISA Kit employs a two-site sandwich ELISA to quantitate UBD in samples. An antibody specific for UBD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Ubiquitin D is a protein encoded by the UBD gene.Using selective cDNA hybridization of a YAC containing the HLA-F locus region on chromosome 6, followed by Southern and Northern blot analyses, Fan et al. (1996) isolated a cDNA, which they called 1F12, encoding a protein similar to diubiquitin. The 1.1-kb transcript was detected only in B-cell lines transformed by Epstein-Barr virus. By subtractive hybridization of dendritic cell (DC) libraries and EST database searching, Bates et al. (1997) obtained a full-length cDNA encoding diubiquitin. The predicted 165-amino acid protein has 2 ubiquitin-like domains, each of which has 2 cysteines. Southern blot analysis indicated that the cDNA represents a single-copy gene.
Background: Ubiquitin D is a protein encoded by the UBD gene.Using selective cDNA hybridization of a YAC containing the HLA-F locus region on chromosome 6, followed by Southern and Northern blot analyses, Fan et al. (1996) isolated a cDNA, which they called 1F12, encoding a protein similar to diubiquitin. The 1.1-kb transcript was detected only in B-cell lines transformed by Epstein-Barr virus. By subtractive hybridization of dendritic cell (DC) libraries and EST database searching, Bates et al. (1997) obtained a full-length cDNA encoding diubiquitin. The predicted 165-amino acid protein has 2 ubiquitin-like domains, each of which has 2 cysteines. Southern blot analysis indicated that the cDNA represents a single-copy gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ubiquitin D
NCBI Official Synonym Full Names
ubiquitin D
NCBI Official Symbol
UBD
NCBI Official Synonym Symbols
FAT10; UBD-3; GABBR1
NCBI Protein Information
ubiquitin D
UniProt Protein Name
Ubiquitin D
UniProt Gene Name
UBD
UniProt Synonym Gene Names
FAT10
UniProt Entry Name
UBD_HUMAN
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Product Notes
The Human UBD ubd (Catalog #AAA330327) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330327 ELISA Kit recognizes Human UBD. It is sometimes possible for the material contained within the vial of "Ubiquitin D (UBD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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