Human Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit | UCP1 elisa kit

Human Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit

Cat. Num. AAA13915

• Gene Names: UCP1; UCP; SLC25A7
• Reactivity: Human
• Synonyms: Uncoupling Protein 1, Mitochondrial (UCP1); N/A; Human Uncoupling Protein 1, Mitochondrial (UCP1) ELISA Kit; SLC25A7; UCP; Thermogenin; Solute carrier family 25 member 7; UCP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of UCP1. No significant cross-reactivity or interference between UCP1 and analogues was observed.
• Sequence Length: 307
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates or other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level UCP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level UCP1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Standard Curve (Sample)

Related Product Information for UCP1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCP1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to UCP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UCP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UCP1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of UCP1 in human tissue homogenates, cell lysates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 11225256
• NCBI GeneID: 7350
• NCBI Accession #: NP_068605.1
• NCBI GenBank Nucleotide #: NM_021833.4
• UniProt Accession #: P25874; Q13218; Q4KMZ3; Q68G66
• Molecular Weight: 33,005 Da
• NCBI Official Full Name: mitochondrial brown fat uncoupling protein 1
• NCBI Official Synonym Full Names: uncoupling protein 1
• NCBI Official Symbol: UCP1
• NCBI Official Synonym Symbols: UCP; SLC25A7
• NCBI Protein Information: mitochondrial brown fat uncoupling protein 1
• UniProt Protein Name: Mitochondrial brown fat uncoupling protein 1
• UniProt Gene Name: UCP1
• UniProt Synonym Gene Names: UCP 1Curated