Standard Curve (Sample)
(Fig.1. Human Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) Standard Curve.)
Human Urokinase-Type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit | PLAUR/uPAR elisa kit
Human Urokinase-Type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit
Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Reactivity
Human
Synonyms
Urokinase-Type Plasminogen Activator Receptor (PLAUR/uPAR); N/A; Human Urokinase-Type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit; PLAUR/uPAR; CD87; UPAR; URKR; monocyte activation antigen Mo3; u-plasminogen activator receptor form 2; PLAUR/uPAR elisa kit
Reactivity
Human
Specificity
Human Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit has high sensitivity and excellent specificity for detection of Human PLAUR/uPAR. No significant cross-reactivity or interference between Human PLAUR/uPAR and analogues was observed.
Sequence Length
335
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) Standard Curve.)
Related Product Information for PLAUR/uPAR elisa kit
Description: This Human Urokinase-type Plasminogen Activator Receptor (PLAUR/uPAR) ELISA Kit employs a two-site sandwich ELISA to quantitate PLAUR/uPAR in samples. An antibody specific for PLAUR/uPAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLAUR/uPAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLAUR/uPAR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLAUR/uPAR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: uPAR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. All three domains are necessary for high affinity binding of the primary ligand, urokinase. It has been possible to express uPAR recombinantly in CHO-cells and S2 cells. 4 out of 5 of the possible glycosylation sites are used in vivo giving the protein a molecular weight of 50-60 kDA. Recently the structure of uPAR was solved by X-ray crystallography in complex with an peptide antagonist and with its native ligand, urokinase.However the components of the plasminogen activation system have been found to be highly expressed in many malignant tumors, indicating that tumors are able to hijack the system, and use it in metastasis. Thus inhibitors of the various components of the plasminogen activation system has been sought as possible anticancer drugs.
Background: uPAR consists of three different domains of the Ly-6/uPAR/alpha-neurotoxin family. All three domains are necessary for high affinity binding of the primary ligand, urokinase. It has been possible to express uPAR recombinantly in CHO-cells and S2 cells. 4 out of 5 of the possible glycosylation sites are used in vivo giving the protein a molecular weight of 50-60 kDA. Recently the structure of uPAR was solved by X-ray crystallography in complex with an peptide antagonist and with its native ligand, urokinase.However the components of the plasminogen activation system have been found to be highly expressed in many malignant tumors, indicating that tumors are able to hijack the system, and use it in metastasis. Thus inhibitors of the various components of the plasminogen activation system has been sought as possible anticancer drugs.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
urokinase plasminogen activator surface receptor isoform 1
NCBI Official Synonym Full Names
plasminogen activator, urokinase receptor
NCBI Official Symbol
PLAUR
NCBI Official Synonym Symbols
CD87; UPAR; URKR; U-PAR
NCBI Protein Information
urokinase plasminogen activator surface receptor
UniProt Protein Name
Urokinase plasminogen activator surface receptor
UniProt Gene Name
PLAUR
UniProt Synonym Gene Names
MO3; UPAR; U-PAR; uPAR
UniProt Entry Name
UPAR_HUMAN
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Product Notes
The Human PLAUR/uPAR plaur (Catalog #AAA331386) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331386 ELISA Kit recognizes Human PLAUR/uPAR. It is sometimes possible for the material contained within the vial of "Urokinase-Type Plasminogen Activator Receptor (PLAUR/uPAR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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