Standard Curve (Sample)
(Fig.1. Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) Standard Curve.)
Human Vitamin K epoxide Reductase Complex Subunit 1 (VKORC1) ELISA Kit | VKORC1 elisa kit
Human Vitamin K epoxide Reductase Complex Subunit 1 (VKORC1) ELISA Kit
Gene Names
VKORC1; VKOR; MST134; MST576; VKCFD2; EDTP308
Reactivity
Human
Synonyms
Vitamin K epoxide Reductase Complex Subunit 1 (VKORC1); N/A; Human Vitamin K epoxide Reductase Complex Subunit 1 (VKORC1) ELISA Kit; VKORC1; EDTP308; FLJ00289; IMAGE3455200; MGC2694; MST134; MST576; VKCFD2; VKOR; phylloquinone epoxide reductase; vitamin K dependent clotting factors deficiency 2; vitamin K1 epoxide reductase (warfarin-sensi; VKORC1 elisa kit
Reactivity
Human
Specificity
Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VKORC1. No significant cross-reactivity or interference between Human VKORC1 and analogues was observed.
Sequence Length
163
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) Standard Curve.)
Related Product Information for VKORC1 elisa kit
Description: This Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VKORC1 in samples. An antibody specific for VKORC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VKORC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VKORC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VKORC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. VKORC1 is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described.
Background: Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. VKORC1 is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
vitamin K epoxide reductase complex subunit 1 isoform 1
NCBI Official Synonym Full Names
vitamin K epoxide reductase complex subunit 1
NCBI Official Symbol
VKORC1
NCBI Official Synonym Symbols
VKOR; MST134; MST576; VKCFD2; EDTP308
NCBI Protein Information
vitamin K epoxide reductase complex subunit 1
UniProt Protein Name
Vitamin K epoxide reductase complex subunit 1
UniProt Gene Name
VKORC1
UniProt Synonym Gene Names
VKOR
UniProt Entry Name
VKOR1_HUMAN
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Product Notes
The Human VKORC1 vkorc1 (Catalog #AAA330278) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330278 ELISA Kit recognizes Human VKORC1. It is sometimes possible for the material contained within the vial of "Vitamin K epoxide Reductase Complex Subunit 1 (VKORC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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