Application Data
(This assay recognizes both recombinant and natural Human VSIG4. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human VSIG4 ELISA Kit | VSIG4 elisa kit
Human VSIG4 ELISA Kit
Reactivity
Human
Synonyms
VSIG4; N/A; Human VSIG4 ELISA Kit; VSIG4 ELISA Kit, Human; V-set and immunoglobulin domain containing 4; CRIg ELISA Kit, Human; Z39IG ELISA Kit, Human; VSIG4 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human VSIG4 concentration in cell lysate.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Lysate
Detection Range
23.44-1500pg/mL
Sensitivity
12.63pg/mL
Conjugation
HRP
Interference
Preparations of APP, FLT4, NEF and GK at 25ng/mL in a mid-range recombinant human VSIG4 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human VSIG4. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Human VSIG4. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for VSIG4 elisa kit
This VSIG4 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human VSIG4 protein in Lysate. It contains recombinant Human VSIG4, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, a V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.
Background: VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, a V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.
References
Vogt, L. et al., 2006, J Clin Invest.116: 2817-2826.
Helmy, K. et al., 2006, Cell. 124:915-927.
Wiesmann, C. et al., 2006, Nature. 444:217-220.
Zang, X. et al., 2006, J Clin Invest. 116: 2590-2593.
Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325.
He, JQ. et al., 2008, Mol Immunol. 45: 4041-4047.
Helmy, K. et al., 2006, Cell. 124:915-927.
Wiesmann, C. et al., 2006, Nature. 444:217-220.
Zang, X. et al., 2006, J Clin Invest. 116: 2590-2593.
Katschke, KJ. et al., 2007, J. Exp. Med. 204:1319-1325.
He, JQ. et al., 2008, Mol Immunol. 45: 4041-4047.
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Product Notes
The Human VSIG4 (Catalog #AAA258446) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258446 ELISA Kit recognizes Human VSIG4. It is sometimes possible for the material contained within the vial of "VSIG4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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