FCM/FACS (Flow Cytometry)
(WNT1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse WNT1 Polyclonal Antibody | anti-WNT1 antibody
WNT1 Antibody (C-term)
Gene Names
WNT1; INT1; OI15; BMND16
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
WNT1, Antibody; WNT1 Antibody (C-term); Proto-oncogene Wnt-1; Proto-oncogene Int-1 homolog; WNT1; INT1; anti-WNT1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This WNT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human WNT1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
260-288
Sequence Length
370
Applicable Applications for anti-WNT1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(WNT1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(WNT1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(WNT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(WNT1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human testis followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the WNT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of WNT1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). WNT1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of WNT1 Antibody (C-term) in mouse heart tissue lysates (35ug/lane). WNT1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-WNT1 antibody
WNT1 is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum.
Product Categories/Family for anti-WNT1 antibody
References
Shuai,X., et.al., Cancer Genet. Cytogenet. 194 (2), 119-124 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40982
NCBI Official Full Name
proto-oncogene Wnt-1
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 1
NCBI Official Symbol
WNT1
NCBI Official Synonym Symbols
INT1; OI15; BMND16
NCBI Protein Information
proto-oncogene Wnt-1
UniProt Protein Name
Proto-oncogene Wnt-1
UniProt Gene Name
WNT1
UniProt Synonym Gene Names
INT1
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Product Notes
The WNT1 wnt1 (Catalog #AAA285377) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 260-288. The WNT1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the WNT1 wnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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