Human WISP1/CCN4 ELISA Kit | WISP1 elisa kit

Human WISP1/CCN4 PicoKine ELISA Kit

Cat. Num. AAA45992

• Gene Names: WISP1; CCN4; WISP1c; WISP1i; WISP1tc
• Reactivity: Human
• Synonyms: WISP1/CCN4; N/A; Human WISP1/CCN4 PicoKine ELISA Kit; WNT1-inducible-signaling pathway protein 1; WNT1 inducible signaling pathway protein 1; CCN family member 4; CCN4; WISP1; WISP1c; WISP1i; WISP1tc; Wnt 1 inducible signaling pathway protein 1; Wnt-1 induced secreted protein; WNT1 induced secreted protein 1; WNT1 inducible-signaling pathway protein 1; WISP1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human WISP1
• Sequence Length: 367
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human WISP1/CCN4 PicoKine ELISA Kit standard curve)

Related Product Information for WISP1 elisa kit

Principle of the assay: human WISP1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for WISP1 has been precoated onto 96-well plates. Standards(NSO, T23-N367) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for WISP1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human WISP1 amount of sample captured in plate.
Background: WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84% of tumors examined compared with patient-matched normal mucosa.

NCBI and Uniprot Product Information

• NCBI GI #: 34098393
• NCBI GeneID: 8840
• UniProt Accession #: O95388; Q5JBS6; Q5JBS7; Q5JBS8; Q9HCS3; A8KAG6; E7EMM5
• Molecular Weight: 21,550 Da
• NCBI Official Full Name: WNT1-inducible-signaling pathway protein 1
• NCBI Official Synonym Full Names: WNT1 inducible signaling pathway protein 1
• NCBI Official Symbol: WISP1
• NCBI Official Synonym Symbols: CCN4; WISP1c; WISP1i; WISP1tc
• NCBI Protein Information: WNT1-inducible-signaling pathway protein 1; CTC-458A3.8; CCN family member 4; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1
• UniProt Protein Name: WNT1-inducible-signaling pathway protein 1
• UniProt Gene Name: WISP1
• UniProt Synonym Gene Names: CCN4; WISP-1
• UniProt Entry Name: WISP1_HUMAN