Standard Curve (Sample)
Human WNT1-Inducible-Signaling Pathway Protein 1 (WISP1) ELISA Kit | WISP1 elisa kit
Human WNT1-Inducible-Signaling Pathway Protein 1 (WISP1) ELISA Kit
Gene Names
CCN4; WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
Reactivity
Human
Synonyms
WNT1-Inducible-Signaling Pathway Protein 1 (WISP1); N/A; Human WNT1-Inducible-Signaling Pathway Protein 1 (WISP1) ELISA Kit; WISP1; CCN4; WISP1c; WISP1i; WISP1tc; WNT1 induced secreted protein 1; Wnt-1 inducible signaling pathway protein 1; wnt-1 signaling pathway protein 1; WISP1 elisa kit
Reactivity
Human
Specificity
Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human WISP1. No significant cross-reactivity or interference between Human WISP1 and analogues was observed.
Sequence Length
367
Assay Type
Sandwich
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for WISP1 elisa kit
Background: WISP1 is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consis ts of six, modular structured, secreted proteins. WISP1 is mainly expressed during organ development and under diseased conditions, such as fibrosis or cancer. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production, thereby representing a highly attractive therapeutical target for future applications.
Principle of the Assay: Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit employs a two-site sandwich ELISA to quantitate WISP1 in samples. An antibody specific for WISP1 has been pre pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WISP1 present is bound by the immobilized antibody. After removing any unbound substances substances, HRP -ConjugateConjugated Human WISP1 detection antib ody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of WISP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human WNT1-inducible-signaling pathway protein 1 (WISP1) ELISA Kit employs a two-site sandwich ELISA to quantitate WISP1 in samples. An antibody specific for WISP1 has been pre pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WISP1 present is bound by the immobilized antibody. After removing any unbound substances substances, HRP -ConjugateConjugated Human WISP1 detection antib ody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of WISP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
WNT1-inducible-signaling pathway protein 1
NCBI Official Synonym Full Names
cellular communication network factor 4
NCBI Official Symbol
CCN4
NCBI Official Synonym Symbols
WISP1; WISP1c; WISP1i; WISP1tc; WISP1-OT1; WISP1-UT1
NCBI Protein Information
WNT1-inducible-signaling pathway protein 1
UniProt Protein Name
WNT1-inducible-signaling pathway protein 1
UniProt Gene Name
WISP1
UniProt Synonym Gene Names
CCN4; WISP-1
UniProt Entry Name
WISP1_HUMAN
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Product Notes
The Human WISP1 wisp1 (Catalog #AAA330259) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330259 ELISA Kit recognizes Human WISP1. It is sometimes possible for the material contained within the vial of "WNT1-Inducible-Signaling Pathway Protein 1 (WISP1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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