Human Xin actin binding repeat containing protein 1 (XIRP1) ELISA Kit | XIRP1 elisa kit
Human Xin actin binding repeat containing protein 1 (XIRP1) ELISA Kit
Gene Names
XIRP1; Xin; CMYA1
Reactivity
Human
Synonyms
Xin actin binding repeat containing protein 1 (XIRP1); N/A; Human Xin actin binding repeat containing protein 1 (XIRP1) ELISA Kit; XIRP1 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for XIRP1 elisa kit
Intended Uses: This XIRP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human XIRP1. This ELISA kit for research use only!
Principle of the Assay||XIRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XIRP1 antibody and an XIRP1-HRP conjugate. The assay sample and buffer are incubated together with XIRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XIRP1 concentration since XIRP1 from samples and XIRP1-HRP conjugate compete for the anti-XIRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by XIRP1 from the sample, fewer sites are left to bind XIRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XIRP1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||XIRP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-XIRP1 antibody and an XIRP1-HRP conjugate. The assay sample and buffer are incubated together with XIRP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the XIRP1 concentration since XIRP1 from samples and XIRP1-HRP conjugate compete for the anti-XIRP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by XIRP1 from the sample, fewer sites are left to bind XIRP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XIRP1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for XIRP1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,273 Da
NCBI Official Full Name
xin actin-binding repeat-containing protein 1 isoform 1
NCBI Official Synonym Full Names
xin actin-binding repeat containing 1
NCBI Official Symbol
XIRP1
NCBI Official Synonym Symbols
Xin; CMYA1
NCBI Protein Information
xin actin-binding repeat-containing protein 1; cardiomyopathy associated 1; cardiomyopathy-associated protein 1
UniProt Protein Name
Xin actin-binding repeat-containing protein 1
UniProt Gene Name
XIRP1
UniProt Synonym Gene Names
CMYA1; XIN
UniProt Entry Name
XIRP1_HUMAN
