Human Zinc finger and BTB domain containing protein 7A (ZBTB7A) ELISA Kit | ZBTB7A elisa kit

Human Zinc finger and BTB domain containing protein 7A (ZBTB7A) ELISA Kit

Cat. Num. AAA247060

• Gene Names: ZBTB7A; LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon
• Reactivity: Human
• Synonyms: Zinc finger and BTB domain containing protein 7A (ZBTB7A); N/A; Human Zinc finger and BTB domain containing protein 7A (ZBTB7A) ELISA Kit; ZBTB7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ZBTB7A. No significant cross-reactivity or interference between ZBTB7A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ZBTB7A and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ZBTB7A elisa kit

Principle of the Assay: ZBTB7A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ZBTB7A antibody and an ZBTB7A-HRP conjugate. The assay sample and buffer are incubated together with ZBTB7A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZBTB7A concentration since ZBTB7A from samples and ZBTB7A-HRP conjugate compete for the anti-ZBTB7A antibody binding site. Since the number of sites is limited, as more sites are occupied by ZBTB7A from the sample, fewer sites are left to bind ZBTB7A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZBTB7A concentration in each sample is interpolated from this standard curve.

Intended Uses: This ZBTB7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZBTB7A. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 7705375
• NCBI GeneID: 51341
• NCBI Accession #: NP_056982.1
• NCBI GenBank Nucleotide #: NM_015898.2
• Molecular Weight: 61,439 Da
• NCBI Official Full Name: zinc finger and BTB domain-containing protein 7A
• NCBI Official Synonym Full Names: zinc finger and BTB domain containing 7A
• NCBI Official Symbol: ZBTB7A
• NCBI Official Synonym Symbols: LRF; FBI1; FBI-1; ZBTB7; ZNF857A; pokemon
• NCBI Protein Information: zinc finger and BTB domain-containing protein 7A; TIP21; lymphoma related factor; zinc finger protein 857A; HIV-1 1st-binding protein 1; TTF-I-interacting peptide 21; factor binding IST protein 1; leukemia/lymphoma-related factor; POK erythroid myeloid ontogenic factor; HIV-1 inducer of short transcripts binding protein; POZ and Krueppel erythroid myeloid ontogenic factor; factor that binds to inducer of short transcripts protein 1; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcripts binding protein
• UniProt Protein Name: Zinc finger and BTB domain-containing protein 7A
• UniProt Gene Name: ZBTB7A
• UniProt Synonym Gene Names: FBI1; LRF; ZBTB7; ZNF857A; FBI-1; POK erythroid myeloid ontogenic factor; Pokemon; TIP21
• UniProt Entry Name: ZBT7A_HUMAN