TNF alpha elispot kit

Human TNF alpha ELISpot Kit

Cat. Num. AAA58638

• Gene Names: TNF; DIF; TNFA; TNFSF2; TNF-alpha
• Reactivity: Human. No cross reactivity with other human cytokines. Cross reacivity with macac TNF-a. No interference with TNFR I and TNFR II
• Synonyms: TNF alpha; N/A; Human TNF alpha ELISpot Kit; TNF-a; TNF alpha elispot kit

• Reactivity: Human. No cross reactivity with other human cytokines. Cross reacivity with macac TNF-a. No interference with TNFR I and TNFR II
• Specificity: The assay recognizes natural Human TNFalpha. To define specificity of this TNFalpha antibody pair, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1beta, IL-2, IL-4, IL-6, IL-10, IL-12, IFNgamma, IL-8 and IL-13). This testing was performed using the equivalent Human TNFalpha antibody pair in an ELISA assay.
• Sequence Length: 233
• Assay Type: Sandwich

Related Product Information for TNF alpha elispot kit

Principle of the Assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Product Categories/Family for TNF alpha elispot kit

Human ELISpot Kit

References

Godard B. et al., Hum. Immunol., 2004; 65(11): 1307-18
Hudak, S. et al., J Immunol.,2002;169(3): 1189-96.

NCBI and Uniprot Product Information

• NCBI GI #: 37210
• NCBI GeneID: 7124
• UniProt Accession #: P01375; O43647; Q9P1Q2; Q9UIV3
• Molecular Weight: 25,644 Da
• NCBI Official Full Name: TNF-alpha
• NCBI Official Synonym Full Names: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: DIF; TNFA; TNFSF2; TNF-alpha
• NCBI Protein Information: tumor necrosis factor
• UniProt Protein Name: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_HUMAN