IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.)
anti-Human, Mouse 4E-BP1 Antibody | anti-4E-BP1 antibody
4E-BP1 (Phospho-Thr69) Antibody
Gene Names
ESPL1; ESP1; SEPA
Reactivity
Human, Mouse
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
4E-BP1; N/A; 4E-BP1 (Phospho-Thr69) Antibody; Caspase-like protein ESPL1; EC 3.4.22.49; ESP1; ESPL1; Extra spindle poles-like 1 protein; KIAA0165; Separin; anti-4E-BP1 antibody
Reactivity
Human, Mouse
Specificity
SEPARASE (Phospho-Ser801) antibody detects endogenous levels of SEPARASE only when phosphorylated at serine 801.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
2120
Applicable Applications for anti-4E-BP1 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human SEPARASE around the phosphorylation site of serine 801 (N-S-SP-P-V).
All Sites
Human: Ser801
Mouse: Ser1189
Mouse: Ser1189
Preparation and Storage
Store at-20 degree C for 1 year.
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.)
IF (Immunofluorescence)
(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using SEPARASE (Phospho-Ser801) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Phospho-Ser801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using SEPARASE (Phospho-Ser801) antibody. Western blot analysis of extracts from 293 cells, treated with EGF (200ng/ml, 30mins), using SEPARASE (Phospho-Ser801) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
Product Categories/Family for anti-4E-BP1 antibody
References
Waizenegger I., Curr. Biol. 12:1368-1378(2002). Nagase T., DNA Res. 3:17-24(1996). Zou H., Science 285:418-422(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
197,745 Da
NCBI Official Full Name
separin
NCBI Official Synonym Full Names
extra spindle pole bodies like 1, separase
NCBI Official Symbol
ESPL1
NCBI Official Synonym Symbols
ESP1; SEPA
NCBI Protein Information
separin
UniProt Protein Name
Separin
UniProt Gene Name
ESPL1
UniProt Synonym Gene Names
ESP1; KIAA0165
Similar Products
Product Notes
The 4E-BP1 espl1 (Catalog #AAA109712) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The 4E-BP1 (Phospho-Thr69) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 4E-BP1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the 4E-BP1 espl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4E-BP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.