WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using AML1 (Phospho-Ser303) Antibody (#A0409).)
anti-Human, Mouse AML1 Antibody | anti-AML1 antibody
AML1 (Phospho-Ser303) Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
AML1; N/A; AML1 (Phospho-Ser303) Antibody; Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor; alpha 2 subunit; Oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer binding protein 2 alpha B subunit; RUN1; RUNX1; Runt-related transcription factor 1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; anti-AML1 antibody
Reactivity
Human, Mouse
Specificity
AML1 (Phospho-Ser303) antibody detects endogenous levels of AML-1/RUNX1 only when phosphorylated at serine 303.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
453
Applicable Applications for anti-AML1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human AML-1/RUNX1 around the phosphorylation site of serine 303 (P-I-SP-P-G).
All Sites
Human: Ser303
Mouse: Ser276
Mouse: Ser276
Preparation and Storage
Store at-20 degree C for 1 year
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using AML1 (Phospho-Ser303) Antibody (#A0409).)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using AML1 (Phospho-Ser303) Antibody (#A0409).)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AML1 (Phospho-Ser303) Antibody (#A0409).)
IHC (Immunohistochemistry)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using AML1 (Phospho-Ser303) Antibody (#A0409).)
References
S Meyers, Mol. Cell. Biol., Oct 1993; 13: 6336-6345. Chaitali Banerjee, PNAS, May 1996; 93: 4968. Jennifer J. Westendorf, Mol. Cell. Biol., Jan 1998; 18: 322-333. Ari Melnick, Blood, Dec 2000; 96: 3939-3947.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,733 Da
NCBI Official Full Name
runt-related transcription factor 1 isoform AML1b
NCBI Official Synonym Full Names
runt related transcription factor 1
NCBI Official Symbol
RUNX1
NCBI Official Synonym Symbols
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
NCBI Protein Information
runt-related transcription factor 1
UniProt Protein Name
Runt-related transcription factor 1
UniProt Gene Name
RUNX1
UniProt Synonym Gene Names
AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
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Product Notes
The AML1 runx1 (Catalog #AAA267389) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The AML1 (Phospho-Ser303) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AML1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AML1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AML1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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