WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using AML1 (Ab-435) antiobdy. The lane on the left is treated with synthesized peptide.)
Rabbit anti-Human AML1 Polyclonal Antibody | anti-AML1 antibody
AML1 (Ab-435) Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
AML1, Antibody; AML1 (Ab-435) Antibody; Acute myeloid leukemia 1 protein; CBF-alpha 2; CBFA2; Core-binding factor; alpha 2 subunit; anti-AML1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total AML1 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
453
Applicable Applications for anti-AML1 antibody
WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized non-phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-S(p)-P-T).
Target Name
AML1
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using AML1 (Ab-435) antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from 293 cells (Lane 2) and Hela cells (Lane 3), using AML1 (Ab-435) antiobdy. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Ab-435) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Ab-435) antibody.)
Related Product Information for anti-AML1 antibody
CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes with the transactivation activity of RUNX1. Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the mouse BLK promoter. Inhibits KAT6B-dependent transcriptional activation.Miyoshi H., Proc. Natl. Acad. Sci. U.S.A. 88:10431-10434(1991).Sacchi N., Genes Chromosomes Cancer 11:226-236(1994).Nucifora G., Blood 81:2728-2734(1993).
Product Categories/Family for anti-AML1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,733 Da
NCBI Official Full Name
runt-related transcription factor 1 isoform AML1b
NCBI Official Synonym Full Names
runt-related transcription factor 1
NCBI Official Symbol
RUNX1
NCBI Official Synonym Symbols
AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
NCBI Protein Information
runt-related transcription factor 1
UniProt Protein Name
Runt-related transcription factor 1
UniProt Gene Name
RUNX1
UniProt Synonym Gene Names
AML1; CBFA2; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B
UniProt Entry Name
RUNX1_HUMAN
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Product Notes
The AML1 runx1 (Catalog #AAA305234) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AML1 (Ab-435) Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AML1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the AML1 runx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AML1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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