WB (Western Blot)
(Western blot using anti-Aquaporin 4 antibody D15107 . Unboiled (A) and boiled (B) mouse skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of D15107 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
anti-Human, Mouse Aquaporin 4 Antibody | anti-AQP4 antibody
Anti-Aquaporin 4 [D15107]
Gene Names
AQP4; MIWC; HMIWC2
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, ELISA
Purity
Protein A affinity purified
Synonyms
Aquaporin 4; N/A; Anti-Aquaporin 4 [D15107]; AQP4; AQP-4; Aquaporin-4; MIWC; WCH4; Mercurial-insensitive water channel; anti-AQP4 antibody
Reactivity
Human, Mouse
Isotype
Mouse IgG2a kappa
Clone Number
D15107
Specificity
This antibody recognizes and binds the extracellular domains of both the M1 and M23 isoforms of human aquaporin 4 (AQP4). It also binds the M23 isoform of mouse aquaporin 4, and does not recognize the M1 isoform of mouse aquaporin 4. AQP4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-AQP4 antibody
IA (Inhibition Assay), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA
Immunogen
The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
WB (Western Blot)
(Western blot using anti-Aquaporin 4 antibody D15107 . Unboiled (A) and boiled (B) mouse skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of D15107 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-Aquaporin 4 antibody D15107 . Unboiled (A) and boiled (B) mouse skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of D15107 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-Aquaporin 4 antibody D15107 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with anti-Aquaporin 4 antibody D15107 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of A431 cells with anti-Aquaporin 4 antibody D15107 . Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix™ coverslips stained with anti-Aquaporin 4 antibody D15107 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Product Categories/Family for anti-AQP4 antibody
References
Miyazaki et al.
J Neuroimmunol. 2013 Jul 15;260(1-2):107-16.
J Neuroimmunol. 2013 Jul 15;260(1-2):107-16.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Aquaporin-4
NCBI Official Synonym Full Names
aquaporin 4
NCBI Official Symbol
AQP4
NCBI Official Synonym Symbols
MIWC; HMIWC2
NCBI Protein Information
aquaporin-4; WCH4; aquaporin type4; aquaporin 4 isoform delta4; mercurial-insensitive water channel
UniProt Protein Name
Aquaporin-4
UniProt Gene Name
AQP4
UniProt Synonym Gene Names
AQP-4; MIWC
UniProt Entry Name
AQP4_HUMAN
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Product Notes
The AQP4 aqp4 (Catalog #AAA72339) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Aquaporin 4 [D15107] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 4 can be used in a range of immunoassay formats including, but not limited to, IA (Inhibition Assay), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the AQP4 aqp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aquaporin 4, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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