WB (Western Blot)
(Sample: mouse lung lysate at 30ug; Primary: Anti-Aquaporin 4 at 1:300; Secondary:HRP conjugated Goat-Anti- rabbit IgG at 1: 5000; Predicted band size:36 kD. Observed band size:34 kD)
Rabbit Aquaporin 4 Polyclonal Antibody
Aquaporin 4
Reactivity
Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Protein A affinity purified.
Synonyms
Aquaporin 4, Antibody; Aquaporin 4; aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; AQP4_HUMAN; Aquaporin-4; AQP-4; anti-Aquaporin 4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A affinity purified.
Form/Format
0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Applicable Applications for anti-Aquaporin 4 antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Conjugation
FITC
Immunogen
KLH conjugated synthetic peptide derived from human AQP4 271-323/323
Positive control
Brain, muscle, heart, kidney, lung, and trachea.
Subcellular location
Membrane; Multi-pass membrane protein.
Preparation and Storage
Store at -20 degree C for one year. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Sample: mouse lung lysate at 30ug; Primary: Anti-Aquaporin 4 at 1:300; Secondary:HRP conjugated Goat-Anti- rabbit IgG at 1: 5000; Predicted band size:36 kD. Observed band size:34 kD)
WB (Western Blot)
(Sample: mouse lung lysate at 30ug; Primary: Anti-Aquaporin 4 at 1:300; Secondary:HRP conjugated Goat-Anti- rabbit IgG at 1: 5000; Predicted band size:36 kD. Observed band size:34 kD)
Application Data
(Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 degree C for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody and DAB staining.)
Application Data
(Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37 degree C for 20 min; Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated 1:200, overnight at 4 degree C, followed by conjugation to the secondary antibody and DAB staining.)
Related Product Information for anti-Aquaporin 4 antibody
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
36 KDa
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Product Notes
The Aquaporin 4 (Catalog #AAA268972) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aquaporin 4 reacts with Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Aquaporin 4 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Aquaporin 4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aquaporin 4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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