IHC (Immunohiostchemistry)
(ITCH (Phospho-Tyr420) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
ITCH Antibody | anti-ITCH antibody
ITCH (Phospho-Tyr420) Antibody
Gene Names
ITCH; AIF4; AIP4; ADMFD; NAPP1
Reactivity
Human (Identities = 100%, Positives = 100%)Mouse (Identities = 100%, Positives = 100%)
Applications
ELISA, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
ITCH; N/A; ITCH (Phospho-Tyr420) Antibody; AIF4; AIP4; atrophin-1-interacting protein 4; EC 6.3.2.-; Itchy homolog E3 ubiquitin protein ligase; NAPP1; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH; anti-ITCH antibody
Reactivity
Human (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Mouse (Identities = 100%, Positives = 100%)
Specificity
ITCH (Phospho-Tyr420) antibody detects endogenous levels of ITCH only when phosphorylated at tyrosine 420.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/mL (varies by lot)
Sequence Length
903
Applicable Applications for anti-ITCH antibody
ELISA, IHC (Immunohistochemistry)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human ITCH around the phosphorylation site of tyrosine 420 (F-I-YP-G-N).
All Sites
Human: Tyr420
Mouse: Tyr381
Mouse: Tyr381
Preparation and Storage
Store at-20 degree C for 1 year.
IHC (Immunohiostchemistry)
(ITCH (Phospho-Tyr420) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
IHC (Immunohiostchemistry)
(ITCH (Phospho-Tyr420) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Application Data
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody.)
Application Data
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using ITCH (Phospho-Tyr420) antibody.)
Product Categories/Family for anti-ITCH antibody
References
Chen X., Genomics 73:238-241(2001). Deloukas P., Nature 414:865-871(2001). Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase Itchy homolog isoform 1
NCBI Official Synonym Full Names
itchy E3 ubiquitin protein ligase
NCBI Official Symbol
ITCH
NCBI Official Synonym Symbols
AIF4; AIP4; ADMFD; NAPP1
NCBI Protein Information
E3 ubiquitin-protein ligase Itchy homolog
UniProt Protein Name
E3 ubiquitin-protein ligase Itchy homolog
UniProt Gene Name
ITCH
UniProt Synonym Gene Names
Itch; AIP4; NAPP1
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Product Notes
The ITCH itch (Catalog #AAA108908) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The ITCH (Phospho-Tyr420) Antibody reacts with Human (Identities = 100%, Positives = 100%) Mouse (Identities = 100%, Positives = 100%) and may cross-react with other species as described in the data sheet. AAA Biotech's ITCH can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the ITCH itch for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITCH, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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