WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 2), using ITCH (Ab-420) antiobdy. The lane on the left is treated with systhesized peptide.)
Rabbit anti-Human, Mouse ITCH Polyclonal Antibody | anti-ITCH antibody
ITCH (Ab-420) Antibody
Gene Names
ITCH; AIF4; AIP4; ADMFD; NAPP1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
ITCH, Antibody; ITCH (Ab-420) Antibody; AIF4; AIP4; atrophin-1-interacting protein 4; EC 6.3.2.-; Itchy homolog E3 ubiquitin protein ligase; anti-ITCH antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total ITCH protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
903
Applicable Applications for anti-ITCH antibody
WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized non-phosphopeptide derived from human ITCH around the phosphorylation site of tyrosine 420 (F-I-Y(p)-G-N).
Target Name
ITCH
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 2), using ITCH (Ab-420) antiobdy. The lane on the left is treated with systhesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells (Lane 2), using ITCH (Ab-420) antiobdy. The lane on the left is treated with systhesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from mouse brain cells, using ITCH (Ab-420) antibody.)
Related Product Information for anti-ITCH antibody
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. It is involved in the control of inflammatory signaling pathways. Is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Ubiquitinates RIPK2 by 'Lys-63'-linked conjugation and influences NOD2-dependent signal transduction pathways. Regulates the transcriptional activity of several transcription factors, and probably plays an important role in the regulation of immune response. Ubiquitinates NFE2 by 'Lys-63' linkages and is implicated in the control of the development of hematopoietic lineages. Critical regulator of T-helper (TH2) cytokine development through its ability to induce JUNB ubiquitination and degradation By similarity. Ubiquitinates SNX9. Ubiquitinates CXCR4 and HGS/HRS and regulates sorting of CXCR4 to the degradative pathway. It is involved in the negative regulation of MAVS-dependent cellular antiviral responses. Ubiquitinates MAVS through 'Lys-48'-linked conjugation resulting in MAVS proteasomal degradation. Involved in the regulation of apoptosis and reactive oxygen species levels through the ubiquitination and proteasomal degradation of TXNIP. Mediates the antiapoptotic activity of epidermal growth factor through the ubiquitination and proteasomal degradation of p15 BID. Targets DTX1 for lysosomal degradation and controls NOTCH1 degradation, in the absence of ligand, through 'Lys-29'-linked polyubiquitination.Chen X., Genomics 73:238-241(2001).Deloukas P., Nature 414:865-871(2001).Wood J.D., Mol. Cell. Neurosci. 11:149-160(1998).
Product Categories/Family for anti-ITCH antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86,498 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase Itchy homolog isoform 1
NCBI Official Synonym Full Names
itchy E3 ubiquitin protein ligase
NCBI Official Symbol
ITCH
NCBI Official Synonym Symbols
AIF4; AIP4; ADMFD; NAPP1
NCBI Protein Information
E3 ubiquitin-protein ligase Itchy homolog
UniProt Protein Name
E3 ubiquitin-protein ligase Itchy homolog
UniProt Gene Name
ITCH
UniProt Synonym Gene Names
Itch; AIP4; NAPP1
UniProt Entry Name
ITCH_HUMAN
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Product Notes
The ITCH itch (Catalog #AAA307532) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ITCH (Ab-420) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ITCH can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ITCH itch for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ITCH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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