WB (Western Blot)
(Western blot using anti-Mesothelin antibody MOR06626 . HeLa (A) (0.1ug/ml) cells lysate, human placenta (B) (0.3ug/ml), and human lung (C) (0.1ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and blots were probed with the chimeric rabbit version of MOR06626 (AAA72331) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
anti-Human, Mouse Mesothelin Antibody | anti-MSLN antibody
Anti-Mesothelin [MOR06626]
Gene Names
MSLN; MPF; SMRP
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified. Protein A affinity purified
Synonyms
Mesothelin; N/A; Anti-Mesothelin [MOR06626]; MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; anti-MSLN antibody
Reactivity
Human, Mouse
Isotype
Rabbit IgG kappa
Clone Number
MOR06626
Specificity
This antibody binds the human and murine mesothelin. Mesothelin is a differentiation antigen present on normal mesothelial cells and overexpressed in several human tumors, including mesothelioma and ovarian and pancreatic adenocarcinoma. Because of its limited expression in normal tissues and overexpression in tumors, mesothelin is a promising candidate for tumor-specific therapy.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MSLN antibody
FCM/FACS (Flow Cytometry), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western blot using anti-Mesothelin antibody MOR06626 . HeLa (A) (0.1ug/ml) cells lysate, human placenta (B) (0.3ug/ml), and human lung (C) (0.1ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and blots were probed with the chimeric rabbit version of MOR06626 (AAA72331) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-Mesothelin antibody MOR06626 . HeLa (A) (0.1ug/ml) cells lysate, human placenta (B) (0.3ug/ml), and human lung (C) (0.1ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and blots were probed with the chimeric rabbit version of MOR06626 (AAA72331) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Mesothelin antibody MOR06626 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MOR06626 (AAA72331) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72331, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of HeLa cells with anti-Mesothelin antibody MOR06626 . Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of MOR06626 (AAA72331) (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, AAA72331, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-MSLN antibody
The original antibody was isolated from a synthetic phage display library (HuCAL) after 3 rounds of panning against whole CHO-A9 and NCI-H226 cells expressing mesothelin.
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
References
Kahnert et al.N/A
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,986 Da
NCBI Official Full Name
mesothelin isoform 1 preproprotein
NCBI Official Synonym Full Names
mesothelin
NCBI Official Symbol
MSLN
NCBI Official Synonym Symbols
MPF; SMRP
NCBI Protein Information
mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
UniProt Protein Name
Mesothelin
UniProt Gene Name
MSLN
UniProt Synonym Gene Names
MPF; MPF
UniProt Entry Name
MSLN_HUMAN
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Product Notes
The MSLN msln (Catalog #AAA72331) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mesothelin [MOR06626] reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mesothelin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the MSLN msln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mesothelin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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