Application Data
(53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
53BP1 Antibody | anti-53BP1 antibody
53BP1 (Phospho-Ser25) Antibody
Gene Names
TP53BP1; TP53; p202; 53BP1; TDRD30; p53BP1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunofluorescence, Immunohistochemistry
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
53BP1; N/A; 53BP1 (Phospho-Ser25) Antibody; P531; TP53B; TP53BP1; TRP53BP1; Tumor suppressor p53-binding protein 1; p53-binding protein 1; anti-53BP1 antibody
Reactivity
Human, Mouse, Rat
Specificity
53BP1 (Phospho-Ser25) antibody detects endogenous levels of 53BP1 only when phosphorylated at serine 25.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1975
Applicable Applications for anti-53BP1 antibody
ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human 53BP1 around the phosphorylation site of serine 25 (E-D-SP-Q-P).
All Sites
Human: Ser25
Mouse: Ser25
Rat: Ser30
Mouse: Ser25
Rat: Ser30
Preparation and Storage
Store at-20 degree C for 1 year.
Application Data
(53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
Application Data
(53BP1 (Phospho-Ser25) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
IHC (Immunohiostchemistry)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody. Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody.)
IHC (Immunohiostchemistry)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody. Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
Product Categories/Family for anti-53BP1 antibody
References
Irene M. Ward. Mol. Cell. Biol., Nov 2005; 25: 10079-10086. Fiona Pryde. J. Cell Sci., May 2005; 118: 2043-2055. Julio C. Morales. J. Biol. Chem., Apr 2003; 278: 14971-14977.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
213,989 Da
NCBI Official Full Name
TP53-binding protein 1 isoform 2
NCBI Official Synonym Full Names
tumor protein p53 binding protein 1
NCBI Official Symbol
TP53BP1
NCBI Official Synonym Symbols
TP53; p202; 53BP1; TDRD30; p53BP1
NCBI Protein Information
TP53-binding protein 1
UniProt Protein Name
TP53-binding protein 1
UniProt Gene Name
TP53BP1
UniProt Synonym Gene Names
p53BP1
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Product Notes
The 53BP1 tp53bp1 (Catalog #AAA109927) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The 53BP1 (Phospho-Ser25) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's 53BP1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IF (Immunofluorescence), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the 53BP1 tp53bp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "53BP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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