Rabbit anti-Human, Mouse 53BP1 Polyclonal Antibody | anti-53BP1 antibody

Rabbit anti-53BP1 Antibody, Affinity Purified

Cat. Num. AAA210853

• Gene Names: TP53BP1; p202; 53BP1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: 53BP1, Antibody; Rabbit anti-53BP1 Antibody, Affinity Purified; p53-binding protein 1; tumor suppressor p53-binding protein 1; tumor protein 53-binding protein, 1; TP53-binding protein 1; p53BP1; p202; 53BP1; TDRD30; Tumor suppressor p53-binding protein 1; tumor protein p53 binding protein 1; anti-53BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-53BP1 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 350 and 400
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-53BP1 antibody AAA210853 (lot AAA210853-7) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)

WB (Western Blot)

(Detection of mouse 53BP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-53BP1 antibody AAA210853 (lot AAA210853-7) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-53BP1 antibody AAA210853 (lot AAA210853-7) used for IP at 6 ug per reaction. 53BP1 was also immunoprecipitated by a previous lot of this antibody (lot AAA210853-6). For blotting immunoprecipitated 53BP1, rabbit anti-recombinant monoclonal antibody [BL-250-1H11] was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry)

(Detection of human 53BP1 by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-53BP1 (Cat. No. AAA210853 lot 7) used at a dilution of 1:5,000 (0.2 ug/ml). Detection: DAB.)

IHC (Immunohistochemistry)

(Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-53BP1 (Cat. No. AAA210853 lot 7) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.)

Related Product Information for anti-53BP1 antibody

53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

Product Categories/Family for anti-53BP1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 5032189
• NCBI GeneID: 7158
• NCBI Accession #: NP_005648.1
• NCBI GenBank Nucleotide #: NM_005657.2
• UniProt Accession #: Q12888; Q2M1Z7; Q4LE46; Q5FWZ3; Q7Z3U4; F8VY86
• Molecular Weight: 213,989 Da
• NCBI Official Full Name: tumor suppressor p53-binding protein 1 isoform 3
• NCBI Official Synonym Full Names: tumor protein p53 binding protein 1
• NCBI Official Symbol: TP53BP1
• NCBI Official Synonym Symbols: p202; 53BP1
• NCBI Protein Information: tumor suppressor p53-binding protein 1; p53BP1; tumor protein 53-binding protein, 1; tumor protein p53-binding protein, 1
• UniProt Protein Name: Tumor suppressor p53-binding protein 1
• UniProt Gene Name: TP53BP1
• UniProt Synonym Gene Names: 53BP1; p53-binding protein 1; p53BP1
• UniProt Entry Name: TP53B_HUMAN