IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using AMPK beta1 (Phospho-Ser181) antibody. Western blot analysis of extracts from COS7 cells treated with PMA (125ng/ml, 30mins), using AMPK beta1 (Phospho-Ser181) antibody.)
AMPK beta1 Antibody | anti-AMPK beta1 antibody
AMPK beta1 (Phospho-Ser181) Antibody
Gene Names
PRKAB1; AMPK; HAMPKb
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
AMPK beta1; N/A; AMPK beta1 (Phospho-Ser181) Antibody; 5'-AMP-activated protein kinase subunit beta-1; AMPK subunit beta-1; AMPKb; PRKAB1; AMPK; anti-AMPK beta1 antibody
Reactivity
Human, Mouse, Rat
Specificity
AMPK beta1 (Phospho-Ser181) antibody detects endogenous levels of AMPK beta1 only when phosphorylated at serine 181.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
270
Applicable Applications for anti-AMPK beta1 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human AMPK beta1 around the phosphorylation site of serine 181 (S-S-SP-P-P).
All Sites
Human: Ser182
Mouse: Ser182
Rat: Ser182
Mouse: Ser182
Rat: Ser182
Preparation and Storage
Store at-20 degree C for 1 year.
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using AMPK beta1 (Phospho-Ser181) antibody. Western blot analysis of extracts from COS7 cells treated with PMA (125ng/ml, 30mins), using AMPK beta1 (Phospho-Ser181) antibody.)
IHC (Immunohiostchemistry)
(Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using AMPK beta1 (Phospho-Ser181) antibody. Western blot analysis of extracts from COS7 cells treated with PMA (125ng/ml, 30mins), using AMPK beta1 (Phospho-Ser181) antibody.)
Application Data
(P-peptide-+)
Application Data
(P-peptide-+)
Product Categories/Family for anti-AMPK beta1 antibody
References
Carling D., Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases. Stapleton D., FEBS Lett. 409:452-456(1997). Yamagata K., Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,382 Da
NCBI Official Full Name
5'-AMP-activated protein kinase subunit beta-1
NCBI Official Synonym Full Names
protein kinase AMP-activated non-catalytic subunit beta 1
NCBI Official Symbol
PRKAB1
NCBI Official Synonym Symbols
AMPK; HAMPKb
NCBI Protein Information
5'-AMP-activated protein kinase subunit beta-1
UniProt Protein Name
5'-AMP-activated protein kinase subunit beta-1
UniProt Gene Name
PRKAB1
UniProt Synonym Gene Names
AMPK; AMPK subunit beta-1; AMPKb
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Product Notes
The AMPK beta1 prkab1 (Catalog #AAA111477) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The AMPK beta1 (Phospho-Ser181) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AMPK beta1 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AMPK beta1 prkab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK beta1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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