WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (line 1), Jurkat cells (line 2) and NIH/3T3 cells (line 3) all treated with PMA (125ng/ml, 30mins), using PKC epsilon (Phospho-Ser729) antibody.)
PKC epsilon Antibody | anti-PKC epsilon antibody
PKC epsilon (Phospho-Ser729) Antibody
Gene Names
PRKCE; PKCE; nPKC-epsilon
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
PKC epsilon; N/A; PKC epsilon (Phospho-Ser729) Antibody; EC 2.7.11.13; KPCE; PKC-epsilon; PKCEA; PRKCE; Protein kinase C; epsilon type; kinase PKC-epsilon; nPKC-epsilon; anti-PKC epsilon antibody
Reactivity
Human, Mouse, Rat
Specificity
PKC epsilon (Phospho-Ser729) antibody detects endogenous levels of PKC epsilon only when phosphorylated at serine 729.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
737
Applicable Applications for anti-PKC epsilon antibody
ELISA, WB (Western Blot)
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PKC epsilon around the phosphorylation site of serine 729 (G-F-SP-Y-F).
All Sites
Human: Ser729
Mouse: Ser729
Rat: Ser729
Mouse: Ser729
Rat: Ser729
Preparation and Storage
Store at-20 degree C for 1 year.
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (line 1), Jurkat cells (line 2) and NIH/3T3 cells (line 3) all treated with PMA (125ng/ml, 30mins), using PKC epsilon (Phospho-Ser729) antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells (line 1), Jurkat cells (line 2) and NIH/3T3 cells (line 3) all treated with PMA (125ng/ml, 30mins), using PKC epsilon (Phospho-Ser729) antibody.)
References
Basta P., Biochim. Biophys. Acta 1132:154-160(1992). Hillier L.W., Nature 434:724-731(2005). Cenni V., Biochem. J. 363:537-545(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,674 Da
NCBI Official Full Name
protein kinase C epsilon type
NCBI Official Synonym Full Names
protein kinase C epsilon
NCBI Official Symbol
PRKCE
NCBI Official Synonym Symbols
PKCE; nPKC-epsilon
NCBI Protein Information
protein kinase C epsilon type
UniProt Protein Name
Protein kinase C epsilon type
UniProt Gene Name
PRKCE
UniProt Synonym Gene Names
PKCE
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Product Notes
The PKC epsilon prkce (Catalog #AAA267511) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PKC epsilon (Phospho-Ser729) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PKC epsilon can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PKC epsilon prkce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC epsilon, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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