IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human PKC epsilon Polyclonal Antibody | anti-PRKCE antibody
PKC epsilon Antibody (N-term)
Gene Names
PRKCE; PKCE; nPKC-epsilon
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PKC epsilon, Antibody; PKC epsilon Antibody (N-term); Protein kinase C epsilon type; nPKC-epsilon; PRKCE; PKCE; anti-PRKCE antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PKC epsilon antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the N-terminal region of human PKC epsilon.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 1.79 (varies by lot)
Sequence Positions
206-236
Sequence Length
737
Applicable Applications for anti-PRKCE antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of PRKCE (arrow) using rabbit polyclonal PKC epsilon Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of PRKCE (arrow) using rabbit polyclonal PKC epsilon Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of anti-PKCepsilon Pab in placenta lysate. PKCepsilon (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
WB (Western Blot)
(Western blot analysis of anti-PKCepsilon Pab in placenta lysate. PKCepsilon (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-PRKCE antibody
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
Product Categories/Family for anti-PRKCE antibody
References
Gray, M.O., et al., J. Biol. Chem. 279(5):3596-3604 (2004).
Ling, M., et al., Exp. Cell Res. 292(1):135-150 (2004).
McJilton, M.A., et al., Oncogene 22(39):7958-7968 (2003).
Robu, V.G., et al., J. Biol. Chem. 278(48):48154-48161 (2003).
Mendez, C.F., et al., J. Biol. Chem. 278(45):44753-44757 (2003).
Ling, M., et al., Exp. Cell Res. 292(1):135-150 (2004).
McJilton, M.A., et al., Oncogene 22(39):7958-7968 (2003).
Robu, V.G., et al., J. Biol. Chem. 278(48):48154-48161 (2003).
Mendez, C.F., et al., J. Biol. Chem. 278(45):44753-44757 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83674
NCBI Official Full Name
protein kinase C epsilon type
NCBI Official Synonym Full Names
protein kinase C, epsilon
NCBI Official Symbol
PRKCE
NCBI Official Synonym Symbols
PKCE; nPKC-epsilon
NCBI Protein Information
protein kinase C epsilon type
UniProt Protein Name
Protein kinase C epsilon type
UniProt Gene Name
PRKCE
UniProt Synonym Gene Names
PKCE
UniProt Entry Name
KPCE_HUMAN
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Product Notes
The PRKCE prkce (Catalog #AAA285085) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 206-236. The PKC epsilon Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PKC epsilon can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the PRKCE prkce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PKC epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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