NMDAR2B elisa kit
NMDAR2B Colorimetric Cell-Based ELISA Kit
Gene Names
GRIN2B; NR3; MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
Reactivity
Human, Mouse, Rat
Synonyms
NMDAR2B; N/A; NMDAR2B Colorimetric Cell-Based ELISA Kit; Glutamate [NMDA] receptor subunit epsilon 2 precursor; GRIN2B; hNR3; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDE2; NME2; NR2B; NR3; NMDAR2B elisa kit
Reactivity
Human, Mouse, Rat
Specificity
The Anti-NMDAR2B Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity. The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
Form/Format
One 96-Well Plate
Sequence Length
1484
Preparation and Storage
Store at 4 degree C.
Related Product Information for NMDAR2B elisa kit
Principle of the Assay: The NMDAR2B Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NMDAR2B protein expression profile in cells. The kit can be used for measuring the relative amounts of NMDAR2B in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NMDAR2B.
Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Product Categories/Family for NMDAR2B elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
165959
NCBI Official Full Name
glutamate receptor ionotropic, NMDA 2B
NCBI Official Synonym Full Names
glutamate ionotropic receptor NMDA type subunit 2B
NCBI Official Symbol
GRIN2B
NCBI Official Synonym Symbols
NR3; MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B
NCBI Protein Information
glutamate receptor ionotropic, NMDA 2B
UniProt Protein Name
Glutamate receptor ionotropic, NMDA 2B
UniProt Gene Name
GRIN2B
UniProt Synonym Gene Names
NMDAR2B; NR2B; NR3; hNR3
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Product Notes
The Human, Mouse, Rat NMDAR2B grin2b (Catalog #AAA315547) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA315547 ELISA Kit recognizes Human, Mouse, Rat NMDAR2B. It is sometimes possible for the material contained within the vial of "NMDAR2B, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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