FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
CD367 Antibody | anti-CD367 antibody
Anti-Hu CD367 PE
Gene Names
CLEC4A; DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
Reactivity
Non-human primates, Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD367; N/A; Anti-Hu CD367 PE; C-type lectin domain family 4 member A; DCIR; CLEC4A; LLIR; anti-CD367 antibody
Reactivity
Non-human primates, Human
Isotype
Mouse IgG1 kappa
Clone Number
9E8
Specificity
The mouse monoclonal antibody 9E8 recognizes an extracellular epitope of human CD367, a type II transmembrane protein of C-lectin family, expressed mainly on antigen presenting cells.
Form/Format
PE
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Applicable Applications for anti-CD367 antibody
FCM/FACS (Flow Cytometry)
Immunogen
CD367 ectodomain fused with human Fc
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human monocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD367 (9E8) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD367 antibody
CD367 is an approximately 20-28 kDa C-type lectin with immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic part. CD367 binds in calcium-dependent manner to mannose, fucose, and weakly also to N-acetylglucosamine. It is expressed on dendritic cells, macrophages, monocytes, B cells, and neutrophils. In rheumatoid arthitis patients CD367 is expressed also on CD4+ T cells. After ligand-mediated triggering, it is internalized by clathrin-dependent endocytosis and contributes to the antigen presentation to CD8+ T cells. It may also be involved in modulation of the antigen presenting cell response.
References
*Klechevsky E, Flamar AL, Cao Y, Blanck JP, Liu M, O'Bar A, Agouna-Deciat O, Klucar P, Thompson-Snipes L, Zurawski S, Reiter Y, Palucka AK, Zurawski G, Banchereau J: Cross-priming CD8+ T cells by targeting antigens to human dendritic cells through DCIR. Blood 2010 Sep 9;116(10):1685-97., URL: https://pubmed.ncbi.nlm.nih.gov/20530286/,*Damasceno D, Andrés MP, van den Bossche WB, Flores-Montero J, de Bruin S, Teodosio C, van Dongen JJ, Orfao A, Almeida J: Expression profile of novel cell surface molecules on different subsets of human peripheral blood antigen-presenting cells. Clin Transl Immunology 2016 Sep 16;5(9):e100., URL: https://pubmed.ncbi.nlm.nih.gov/27766148/,*Autenrieth SE, Grimm S, Rittig SM, Grünebach F, Gouttefangeas C, Bühring HJ: Profiling of primary peripheral blood- and monocyte-derived dendritic cells using monoclonal antibodies from the HLDA10 workshop in Wollongong, Australia. Clin Transl Immunology 2015 Nov 13;4(11):e50., URL: https://pubmed.ncbi.nlm.nih.gov/26682057/,
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
237
NCBI Official Full Name
C-type lectin domain family 4 member A
NCBI Official Synonym Full Names
C-type lectin domain family 4, member A
NCBI Official Symbol
CLEC4A
NCBI Official Synonym Symbols
DCIR; LLIR; DDB27; CLECSF6; HDCGC13P
NCBI Protein Information
C-type lectin domain family 4 member A; C-type lectin DDB27; lectin-like immunoreceptor; dendritic cell immunoreceptor; C-type lectin superfamily member 6; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 6
UniProt Protein Name
C-type lectin domain family 4 member A
UniProt Gene Name
CLEC4A
UniProt Synonym Gene Names
CLECSF6; DCIR; LLIR
UniProt Entry Name
CLC4A_HUMAN
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Product Notes
The CD367 clec4a (Catalog #AAA129255) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD367 PE reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD367 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD367 clec4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD367, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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