WB (Western Blot)
( Western Blot using anti-VEGF antibody Rat skin lysate was resolved on a 10% SDS PAGE gel and blots probed with at 1.0 ug/ml before being detected by a secondary antibody. The expected band size (27 kDa) is seen along with bands corresponding to dimers.)
VEGF recombinant antibody
Anti-VEGF [A.4.6.1]
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation, ELISA
Purity
Protein A Affinity Purified
Synonyms
VEGF; N/A; Anti-VEGF [A.4.6.1]; Vascular Endothelial Growth Factor; VEGF-A; VEGFA; Vascular Permeability Factor; VPF; VEGF recombinant antibody
Reactivity
Human, Rat
Isotype
Mouse IgG1, kappa
Clone Number
A.4.6.1
Specificity
Binds native and reduced human VEGF (isoform 165), with a Kd of 0.8 nm determined for the original clone.
Purity/Purification
Protein A Affinity Purified
Sequence Length
232
Applicable Applications for VEGF recombinant antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), Blocking, WB (Western Blot), IP (Immunoprecipitation), ELISA
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Recombinant human VEGF.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
( Western Blot using anti-VEGF antibody Rat skin lysate was resolved on a 10% SDS PAGE gel and blots probed with at 1.0 ug/ml before being detected by a secondary antibody. The expected band size (27 kDa) is seen along with bands corresponding to dimers.)
WB (Western Blot)
( Western Blot using anti-VEGF antibody Rat skin lysate was resolved on a 10% SDS PAGE gel and blots probed with at 1.0 ug/ml before being detected by a secondary antibody. The expected band size (27 kDa) is seen along with bands corresponding to dimers.)
IHC (Immunohiostchemistry)
( Immunohistochemical staining of rat skin tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat skin tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 was used to stain samples at a concentration of 5 ug/ml.)
IHC (Immunohiostchemistry)
( Immunohistochemical staining of rat skin tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat skin tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 was used to stain samples at a concentration of 5 ug/ml.)
IHC (Immunohistochemistry)
( Immunohistochemical staining of rat brain tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat brain tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 was used to stain samples at a concentration of 5 ug/ml.)
IHC (Immunohistochemistry)
( Immunohistochemical staining of rat brain tissue using anti-VEGF antibody A.4.6.1 Anti-VEGF staining of formaldehyde fixed paraffin embedded rat brain tissue, at 40x magnification. The mouse IgG1-chimeric version of A.4.6.1 was used to stain samples at a concentration of 5 ug/ml.)
Related Product Information for VEGF recombinant antibody
Recombinant monoclonal antibody to VEGF. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma A.4.6.1.
Product Categories/Family for VEGF recombinant antibody
References
Kim et al. 1992 The Vascular Endothelial Growth Factor Proteins: Identification of biologically relelvant regions by neutralizing monoclonal antibodies. Growth Factors. 1992; 7(1):53-64. PMID:1380254
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
34,406 Da
NCBI Official Full Name
Vascular endothelial growth factor A
NCBI Official Synonym Full Names
vascular endothelial growth factor A
NCBI Official Symbol
VEGFA
NCBI Official Synonym Symbols
VPF; VEGF; MVCD1
NCBI Protein Information
vascular endothelial growth factor A
UniProt Protein Name
Vascular endothelial growth factor A
UniProt Gene Name
VEGFA
UniProt Synonym Gene Names
VEGF; VEGF-A; VPF
UniProt Entry Name
VEGFA_HUMAN
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Product Notes
The VEGF vegfa (Catalog #AAA71951) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-VEGF [A.4.6.1] reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's VEGF can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), Blocking, WB (Western Blot), IP (Immunoprecipitation), ELISA. Researchers should empirically determine the suitability of the VEGF vegfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VEGF, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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