IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
alpha 5 beta 1 Integrin Recombinant Antibody
Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Purity
Protein A Affinity Purified
Synonyms
alpha 5 beta 1 Integrin; N/A; Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]; Fibronectin receptor; CD49 antigen-like family member E; Integrin alpha-F; VLA-5; CD49e.; alpha 5 beta 1 Integrin recombinant antibody
Reactivity
Human
Isotype
Rabbit IgG, kappa
Clone Number
M200 (Volociximab)
Specificity
The antibody binds to human a5beta1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.
Purity/Purification
Protein A Affinity Purified
Applicable Applications for alpha 5 beta 1 Integrin recombinant antibody
IHC (Immunohistochemistry), Blocking, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Heterodimeric a5beta1Fc
Chimeric Use Statement
NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
( Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
( Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab) PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
( Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab) PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Related Product Information for alpha 5 beta 1 Integrin recombinant antibody
Recombinant monoclonal antibody to alpha 5 beta 1 Integrin. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma M200 (Volociximab). NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for alpha 5 beta 1 Integrin recombinant antibody
References
Ramakrishnan et al. Preclinical evaluation of an anti-alpha5beta1 integrin antibody as a novel anti-angiogenic agent. J Exp Ther Oncol. 2006; 5(4):273-86. PMID:17024968
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Product Notes
The alpha 5 beta 1 Integrin (Catalog #AAA71955) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's alpha 5 beta 1 Integrin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), Blocking, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the alpha 5 beta 1 Integrin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha 5 beta 1 Integrin, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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