IF (Immunofluorescence)
(Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab) (AAA72236) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (AAA72236-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72236-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
Mouse anti-Human alpha 5 beta 1 Integrin Antibody | anti-IgG1 antibody
Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)], Mouse IgG1
Gene Names
ITGA5; FNRA; CD49e; VLA5A
Reactivity
Human
Applications
Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
Purity
Protein A affinity purified
Synonyms
alpha 5 beta 1 Integrin; N/A; Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)], Mouse IgG1; Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)]; Fibronectin receptor; CD49 antigen-like family member E; Integrin alpha-F; VLA-5; CD49e; anti-IgG1 antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG1, Kappa
Clone Number
M200 (Volociximab)
Specificity
The antibody binds to human alpha5beta1 integrin with a Kd of 0.367 nM (determined by Biacore), an EC50 of 0.2 nM and an IC50 of 2.3 nM.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-IgG1 antibody
IHC (Immunohistochemistry), Blocking, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
Immunogen
Heterodimeric alpha5beta1Fcfusion protein
Chimeric Use Statement
This is a research-grade biosimilar. This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG4 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
IF (Immunofluorescence)
(Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab) (AAA72236) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (AAA72236-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72236-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluoresence staining of fixed HeLa cells with anti-alpha 5 beta 1 Integrin antibody M200 (Volociximab) (AAA72236) Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of M200 (AAA72236-23.0) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic and membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom AAA72236-23.0, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab) (AAA72236) PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (AAA72236-23.0, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-alpha 5 beta 1 Integrin M200 (Volociximab) (AAA72236) PBMC's (A) and U93 cells (B) were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of M200 (Volociximab) (AAA72236-23.0, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.)
Related Product Information for anti-IgG1 antibody
The antibody binds specifically to alpha5beta1 integrin, which is part of the superfamily of glycoprotein transmembrane receptors for ligands such as fibronectin, vitronectin, laminins and collagens. The alpha5beta1 integrin receptor plays a key role in cellular processes such as inflammation, cell proliferation, angiogenesis and tumour metastasis. Comprising the variable domain of the original mouse antibody, a chimeric mouse:human IgG4 version of this antibody has been tested in vivo: In cynomolgus monkeys with laser-induced choroidal neovascularization (CNV), in rabbit models and in human volunteers. The antibody reached Phase II clinical trials with PDL Biopharma and Biogen-Idec.
Product Categories/Family for anti-IgG1 antibody
References
Ramakrishnan et al. Preclinical evaluation of an anti-alpha5beta1 integrin antibody as a novel anti-angiogenic agent. J Exp Ther Oncol. 2006;5(4):273-86. PMID:17024968
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
114,536 Da
NCBI Official Full Name
integrin alpha-5
NCBI Official Synonym Full Names
integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
NCBI Official Symbol
ITGA5
NCBI Official Synonym Symbols
FNRA; CD49e; VLA5A
NCBI Protein Information
integrin alpha-5; VLA-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; very late activation protein 5, alpha subunit
UniProt Protein Name
Integrin alpha-5
UniProt Gene Name
ITGA5
UniProt Synonym Gene Names
FNRA
UniProt Entry Name
ITA5_HUMAN
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Product Notes
The IgG1 itga5 (Catalog #AAA72236) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-alpha 5 beta 1 Integrin [M200 (Volociximab)], Mouse IgG1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's alpha 5 beta 1 Integrin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), Blocking, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the IgG1 itga5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha 5 beta 1 Integrin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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