Standard Curve (Sample)
Human Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) RTU ELISA Kit | SPTLC1 rtu elisa kit
Human Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) ELISA Kit
Gene Names
SPTLC1; HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1
Reactivity
Human
Synonyms
Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1); N/A; Human Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1) ELISA Kit; HSAN; HSAN1; HSN1; LBC1; LCB1; SPT1; SPTI; Hereditary Sensory Neuropathy,Type 1; Long chain base biosynthesis protein 1; Serine-palmitoyl-CoA transferase 1; SPTLC1 rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SPTLC1. No significant cross-reactivity or interference between SPTLC1 and analogues was observed.
Sequence Length
513
Assay Type
Quantitative Sandwich
Samples
Human tissue homogenates or other biological fluids
Detection Range
78.1-5000pg/mL
Sensitivity
< 27ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPTLC1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPTLC1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPTLC1 rtu elisa kit
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPTLC1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPTLC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPTLC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SPTLC1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPTLC1 in human tissue homogenates or other biological fluids.
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPTLC1 in human tissue homogenates or other biological fluids.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16,073 Da
NCBI Official Full Name
serine palmitoyltransferase 1 isoform c
NCBI Official Synonym Full Names
serine palmitoyltransferase long chain base subunit 1
NCBI Official Symbol
SPTLC1
NCBI Official Synonym Symbols
HSN1; LBC1; LCB1; SPT1; SPTI; HSAN1
NCBI Protein Information
serine palmitoyltransferase 1
UniProt Protein Name
Serine palmitoyltransferase 1
UniProt Gene Name
SPTLC1
UniProt Synonym Gene Names
LCB1; LCB 1; SPT 1; SPT1
Similar Products
Product Notes
The Human SPTLC1 sptlc1 (Catalog #AAA219523) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA219523 RTU ELISA Kit recognizes Human SPTLC1. It is sometimes possible for the material contained within the vial of "Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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