Human IL-28B ELISA Kit | IFNL3 elisa kit

Human IL-28B ELISA Kit

Cat. Num. AAA263052

• Gene Names: IFNL3; IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• Reactivity: Human
• Synonyms: IL-28B; N/A; Human IL-28B ELISA Kit; IL28B; IL28C; ZCYTO22; Interferon lambda-3; IFN-lambda-3; Cytokine Zcyto22; Interleukin-28B; Interleukin-28C; IL-28C; IFNL3 elisa kit

• Reactivity: Human
• Specificity: The Human IL-28B ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-28B proteins within the range of 31.2 pg/ml - 2000 pg/ml.
• Sequence Length: 196
• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <2 pg/ml

• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

Related Product Information for IFNL3 elisa kit

Principle of the Assay: The Human IL-28B ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28B in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human IL-28B coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-28B present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-28B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-28B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: IL-28 belongs to the IFN lambda family, a novel family of cytokines within the IL-10 superfamily. The three members of this family are IL-29 (IFN lambda 1), IL-28A (IFN lambda 2), and IL-28B (IFN lambda 3), and are also known as the type III Interferons. An active IL-29 gene is absent in mice, and sequence homology between mouse IL-28A and B is 97%. Expression of these cytokines has been observed in a wide variety of cells in response to viral infection and in response to TLR3 or TLR9 ligands. The IFN lambda family signals through a heterodimeric receptor of which one subunit, IL-10R2, is shared with other members of the superfamily. The second subunit, IFN lambda R1 or IL-28R alpha, is unique to the IFN lambdas. Signaling occurs through the Jak/STAT pathway in a similar manner to signaling by the type I IFNs (IFN alpha/beta) and activates many of the same genes despite low sequence homology between the cytokines and receptors in the two families. Both IFN families display antiviral, antitumor, and antiproliferative effects, making the IFN lambdas potential alternatives to IFN-alpha for anticancer and antiviral therapies. Unlike the type I IFNs, which are able to stimulate most cells, response to IFN lambda stimulation appears to be limited to dendritic, epithelial, and some tumor cells. Another notable difference is the ability of the IFN lambda stimulation to drive dendritic cells towards the production of CD4+CD25+FoxP3+ regulatory T-cells, suggesting a possible immunoregulatory role.

NCBI and Uniprot Product Information

• NCBI GI #: 28144901
• NCBI GeneID: 282617
• NCBI Accession #: NP_742151.2
• NCBI GenBank Nucleotide #: NM_172139.3
• UniProt Accession #: Q8IZI9; Q6VN56; Q7Z4J3; Q8IWL6; A2BDE1
• Molecular Weight: 21,706 Da
• NCBI Official Full Name: interferon lambda-3 isoform 2
• NCBI Official Synonym Full Names: interferon lambda 3
• NCBI Official Symbol: IFNL3
• NCBI Official Synonym Symbols: IL28B; IL28C; IL-28B; IL-28C; IFN-lambda-3; IFN-lambda-4
• NCBI Protein Information: interferon lambda-3
• UniProt Protein Name: Interferon lambda-3
• UniProt Gene Name: IFNL3
• UniProt Synonym Gene Names: IL28B; IL28C; ZCYTO22; IFN-lambda-3; IL-28B; IL-28C
• UniProt Entry Name: IFNL3_HUMAN