Bovine insulin-like growth factor 2 (somatomedin A) ELISA Kit | IGF2 elisa kit

Bovine Insulin-like growth factor II, IGF2 ELISA Kit

Cat. Num. AAA112417

• Reactivity: Bovine
• Synonyms: insulin-like growth factor 2 (somatomedin A); N/A; Bovine Insulin-like growth factor II, IGF2 ELISA Kit; Bovine Insulin-like growth factor II (IGF2) ELISA kit; C11orf43; FLJ22066; FLJ44734; INSIGF; pp9974; OTTHUMP00000011018; OTTHUMP00000011157; insulin-like growth factor 2; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like; IGF2 elisa kit

• Reactivity: Bovine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bovine IGF2. No significant cross-reactivity or interference between Bovine IGF2 and analogues was observed.
• Sequence Length: 179
• Samples: Serum, plasma
• Assay Type: Sandwich
• Detection Range: 31.25 ng/ml-2000 ng/ml
• Sensitivity: 7.81 ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Biosynthesis/Metabolism
• Protein Biological Process 2: glyconeogenesis and glycometabolism
• Protein Biological Process 3: Carbohydrate metabolism
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for IGF2 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IGF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110350675
• NCBI GeneID: 281240
• NCBI Accession #: NP_776512.2
• NCBI GenBank Nucleotide #: NM_174087.3
• UniProt Accession #: P07456; Q2HJN0; A0A5F2
• Molecular Weight: 19,682 Da
• NCBI Official Full Name: insulin-like growth factor II
• NCBI Official Synonym Full Names: insulin-like growth factor 2 (somatomedin A)
• NCBI Official Symbol: IGF2
• NCBI Protein Information: insulin-like growth factor II; IGF-II; erythrotropin
• UniProt Protein Name: Insulin-like growth factor II
• UniProt Gene Name: IGF2
• UniProt Synonym Gene Names: IGF-II
• UniProt Entry Name: IGF2_BOVIN