Standard Curve (Sample)
Rat insulin-like growth factor 2 (somatomedin A) ELISA Kit | IGF2 elisa kit
Rat Insulin-like growth factor 2, IGF-2 ELISA Kit
Gene Names
Igf2; IGFII; RNIGF2
Reactivity
Rat
Synonyms
insulin-like growth factor 2 (somatomedin A); N/A; Rat Insulin-like growth factor 2, IGF-2 ELISA Kit; Rat Insulin-like growth factor 2; IGF-2 ELISA Kit; C11orf43; FLJ22066; FLJ44734; INSIGF; pp9974; OTTHUMP00000011018; OTTHUMP00000011157; insulin-like growth factor 2; insulin-like growth factor II; insulin-like growth factor type 2; putative insulin-like; IGF2 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat IGF2. No significant cross-reactivity or interference between Rat IGF2 and analogues was observed.
Sequence Length
180
Detection Range
78 pg/ml-5000 pg/ml
Sensitivity
19.5 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
glyconeogenesis and glycometabolism
Protein Biological Process 3
Carbohydrate metabolism
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for IGF2 elisa kit
Introduction: Insulin-like growth factor 2 (IGF-2) is one of three protein hormones that share structural similarity to insulin. In humans, the IGF2 gene is located on chromosome 11p15.5, a region which contains numerous imprinted genes. In mice this homologous region is found at distal chromosome 7. In both organisms, Igf2 is imprinted, with expression resulting favourably from the paternally inherited allele. IGF-2 exerts its effects by binding to the IGF-1 receptor. IGF2 may also bind to the IGF-2 receptor (also called the cation-independent mannose 6-phosphate receptor), which acts as a signalling antagonist; that is, to prevent IGF2 responses.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for IGF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for IGF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IGF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IGF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,086 Da
NCBI Official Full Name
insulin-like growth factor II isoform 2 preproprotein
NCBI Official Synonym Full Names
insulin-like growth factor 2
NCBI Official Symbol
Igf2
NCBI Official Synonym Symbols
IGFII; RNIGF2
NCBI Protein Information
insulin-like growth factor II; MSA; IGF-II; multiplication-stimulating activity; insulin-like growth factor II (IGF-II); multiplication-stimulating polypeptide; Insulin-like growth factor II (somatomedin A); insulin-like growth factor II isoform 2 variant 4 preprotein
UniProt Protein Name
Insulin-like growth factor II
UniProt Gene Name
Igf2
UniProt Synonym Gene Names
Igf-2; IGF-II; MSA
UniProt Entry Name
IGF2_RAT
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Product Notes
The Rat IGF2 igf2 (Catalog #AAA79953) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA79953 ELISA Kit recognizes Rat IGF2. It is sometimes possible for the material contained within the vial of "insulin-like growth factor 2 (somatomedin A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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