IgG1 Fc recombinant protein

Recombinant Human IgG1 Fc Protein

Cat. Num. AAA173559

• Purity: > 92 % as determined by reducing SDS-PAGE
• Synonyms: IgG1 Fc; N/A; Recombinant Human IgG1 Fc Protein; g gamma-1 chain C region; IGHG1; IgG1 Fc recombinant protein

• Host: CHO Cells
• Purity/Purification: > 92 % as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Glu 99-Lys 330
• Sequence Length: 329

• Species: Human
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Reconstitution: We recommend that the vial be centrifuged to prior to opening to bring contents to the bottom. Do not mix by vortex.; It is recommended that the sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis
• Preparation and Storage: Lyophilized protein should be stored at < -20°C. Reconstituted protein solution can be stored at 4°C to 7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for IgG1 Fc recombinant protein

Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcgammaR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 184747
• Molecular Weight: Molecular Mass: 26 kDa; AP Molecular Mass: 34 kDa
• NCBI Official Full Name: immunoglobulin gamma-1 heavy chain constant region, partial
• UniProt Protein Name: Ig gamma-1 chain C region
• UniProt Gene Name: IGHG1