Application Data
IKK epsilon recombinant protein
IKK epsilon, Active
Gene Names
IKBKE; IKKE; IKKI; IKK-E; IKK-i
Applications
Western Blot
Synonyms
IKK epsilon; N/A; IKK epsilon, Active; IKK epsilon recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
Full length
Sequence Length
3492
Applicable Applications for IKK epsilon recombinant protein
WB (Western Blot), Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for IKK epsilon recombinant protein
Description: Full length recombinant human IKK epsilon was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: IKK epsilon is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways that has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (1). IKK-epsilon is a part of a novel PMA-inducible I-kappa-B kinase complex which play an essential role for an in triggering the host antiviral response to viral infection. IKKE plays a critical role in the IFN-inducible antiviral transcriptional response (2).
Scientific Background: IKK epsilon is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways that has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (1). IKK-epsilon is a part of a novel PMA-inducible I-kappa-B kinase complex which play an essential role for an in triggering the host antiviral response to viral infection. IKKE plays a critical role in the IFN-inducible antiviral transcriptional response (2).
Product Categories/Family for IKK epsilon recombinant protein
References
1. Hutti, J. E.et.al: Phosphorylation of the tumor suppressor CYLD by the breast cancer oncogene IKK-epsilon promotes cell transformation. Molec. Cell 34: 461-472, 2009. 2. tenOever, B. R. et.al: Multiple functions of the IKK-related kinase IKK-epsilon in interferon-mediated antiviral immunity. Science 315: 1274-1278, 2007.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110 kDa
NCBI Official Full Name
Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA
NCBI Official Synonym Full Names
inhibitor of nuclear factor kappa B kinase subunit epsilon
NCBI Official Symbol
IKBKE
NCBI Official Synonym Symbols
IKKE; IKKI; IKK-E; IKK-i
NCBI Protein Information
inhibitor of nuclear factor kappa-B kinase subunit epsilon
UniProt Protein Name
Inhibitor of nuclear factor kappa-B kinase subunit epsilon
UniProt Gene Name
IKBKE
UniProt Synonym Gene Names
IKKE; IKKI; KIAA0151; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i
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Product Notes
The IKK epsilon ikbke (Catalog #AAA72990) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Full length. AAA Biotech's IKK epsilon can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the IKK epsilon ikbke for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IKK epsilon, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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