Interleukin-4 ELISA Kit | IL-4 elisa kit

Interleukin-4 (IL-4)

Cat. Num. AAA77847

• Gene Names: IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• Synonyms: Interleukin-4; N/A; Interleukin-4 (IL-4); IL-4 elisa kit

• Specificity: This sandwich ELISA recognizes both natural and recombinant human IL-4. This kit exhibits no detectable cross-reactivity with human; SAA, EGF, IL-8, IL- 16, MCAF, MCP-3, TNF-gamma, M-CSF, GM-CSF, TGF-beta1, RANTES, FGF, MIP-1alpha, CRP, EPO, IFN-gamma.
• Sequence Length: 137
• Samples: Serum, plasma, Cell Culture Supernatant, and other biological fluids
• Assay Type: Sandwich
• Sensitivity: The minimum detectable dose of IL-4 was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-4 using a standard curve generated with Calibrator Diluent I is 6.6 pg/mL and using Calibrator Diluent II is 4.3 pg/mL.

• Intra-assay Precision: To determine within-run precision, three different samples of known concentration were assayed by replicates of 20 in 1 assay.
• Inter-assay Precision: To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
• Intended Uses: This Human Interleukin 4 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 4 (IL-4) concentrations in serum, plasma, cell culture supernatant, and other biological fluids. This kit is intended for LABORATORY RESEARCH USE ONLY and is not for use in therapeutic procedures.

Standard Curve (Sample)

Related Product Information for IL-4 elisa kit

Principle of the assay: This IL-4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a polyclonal antibody specific to IL-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for IL-4 and incubated. IL-4 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound IL-4 and other components of the sample. In order to quantitatively determine the amount of IL-4 present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each have a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-4, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzymesubstrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm.

Product Categories/Family for IL-4 elisa kit

ELISA Kits for Human Cytokines and Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 27477092
• NCBI GeneID: 3565
• NCBI Accession #: NP_758858.1
• NCBI GenBank Nucleotide #: NM_172348.2
• Molecular Weight: 15,797 Da
• NCBI Official Full Name: interleukin-4 isoform 2
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: IL4
• NCBI Official Synonym Symbols: BSF1; IL-4; BCGF1; BSF-1; BCGF-1
• NCBI Protein Information: interleukin-4; B cell growth factor 1; B_cell stimulatory factor 1; binetrakin; interleukin 4 variant 2; lymphocyte stimulatory factor 1; pitrakinra
• UniProt Protein Name: Interleukin-4
• UniProt Gene Name: IL4
• UniProt Entry Name: IL4_HUMAN